BORG1 Antibody - #DF3908

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製品: BORG1 Antibody
カタログ: DF3908
タンパク質の説明: Rabbit polyclonal antibody to BORG1
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog
分子量: 28 KD(Observed); 22kD(Calculated).
ユニプロット: O14613
RRID: AB_2836261

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#DF3908-BP

BORG1 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
BORG1 Antibody detects endogenous levels of total BORG1.
RRID:
AB_2836261
引用形式: Affinity Biosciences Cat# DF3908, RRID:AB_2836261.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Binder of Rho GTPases 1; BORG1; BORG1_HUMAN; CDC42 effector protein (Rho GTPase binding) 2; Cdc42 effector protein 2; Cdc42ep2; CEP2; CRIB-containing BOGR1 protein;

免疫原

免疫原:

A synthesized peptide derived from human BORG1, corresponding to a region within N-terminal amino acids.

Uniprot:

O14613(BORG1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CDC42EP2(10435)
発現特異性:
O14613 BORG1_HUMAN:

Highly expressed in the heart. Weakly expressed in the pancreas and liver.

タンパク質配列:
MSTKVPIYLKRGSRKGKKEKLRDLLSSDMISPPLGDFRHTIHIGSGGGSDMFGDISFLQGKFHLLPGTMVEGPEEDGTFDLPFQFTRTATVCGRELPDGPSPLLKNAISLPVIGGPQALTLPTAQAPPKPPRLHLETPQPSPQEGGSVDIWRIPETGSPNSGLTPESGAEEPFLSNASSLLSLHVDLGPSILDDVLQIMDQDLDSMQIPT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Zebrafish
88
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts in a CDC42-dependent manner.

細胞の位置付け:

Endomembrane system>Peripheral membrane protein. Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in the heart. Weakly expressed in the pancreas and liver.

タンパク質ファミリー:

The CRIB domain mediates interaction with CDC42.

Belongs to the BORG/CEP family.

参考文献

1). Cdc42ep1 regulated Fibronectin expression in the TGF-β/Cdc42 signaling pathway during epithelial–mesenchymal transition induced fibrosis. Genes & Diseases, 2026 [IF=9.4]

Application: WB    Species: Mouse    Sample:

Figure S5 Cdc42 interacted with Cdc42ep1 in both BOO mice and TGF-β-treated HUC. (A) Immunohistochemistry images showed the distribution of protein level for Cdc42, Cdc42ep1, Cdc42ep2, Cdc42ep3, Cdc42ep4, and Cdc42ep5 in mouse bladder samples (bar, 500 μm). (B) Co-immunoprecipitation suggested that Cdc42 interacted with Cdc42ep1 in the BOO group. (C) Co-immunoprecipitation suggested that Cdc42 interacted with Cdc42ep1 and Fibronectin in TGF-β-treated HUC. BOO, bladder outlet obstruction; HUC, human urothelial cell line; EMT, epithelial–mesenchymal transition.

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