CNOT4 Antibody - #DF3907
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3907, RRID:AB_2836260.
折りたたみ／展開
CCR4 NOT transcription complex subunit 4; CCR4-associated factor 4; CCR4-NOT transcription complex subunit 4; CLONE243; CNOT 4; Cnot4; CNOT4_HUMAN; E3 ubiquitin-protein ligase CNOT4; NOT 4; NOT4 (negative regulator of transcription 4 yeast) homolog; NOT4; NOT4H; Potential transcriptional repressor NOT4Hp;
免疫原
- O95628 CNOT4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPEDPAVYKPLSQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRLADPEVLKRPEYFGKFGKIHKVVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKNMQCPKPDCMYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQRYDTPIDKPSDSLSIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPPFPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVAGEEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95628 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| T299 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T434 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S440 | Phosphorylation | Uniprot | |
| T443 | Phosphorylation | Uniprot | |
| R475 | Methylation | Uniprot | |
| R483 | Methylation | Uniprot | |
| Y486 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| R497 | Methylation | Uniprot | |
| R505 | Methylation | Uniprot |
研究背景
Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins. Involved in activation of the JAK/STAT pathway. Catalyzes ubiquitination of methylated RBM15.
Autoubiquitinated.
Cytoplasm. Nucleus.
Interacts with CNOT1 via its C-terminus but does not stably associate with the CCR4-NOT complex. Binds E2 ubiquitin ligases via its RING domain. Interacts (via RING domain) with UBE2D2.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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