COMT Antibody - #DF3905
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製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3905, RRID:AB_2836258.
折りたたみ／展開
Catechol O methyltransferase; Catechol O-methyltransferase; COMT; COMT_HUMAN; EC 2.1.1.6;
免疫原
- P21964 COMT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPEAPPLLLAAVLLGLVLLVVLLLLLRHWGWGLCLIGWNEFILQPIHNLLMGDTKEQRILNHVLQHAEPGNAQSVLEAIDTYCEQKEWAMNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPGARLITIEINPDCAAITQRMVDFAGVKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLEYREVVDGLEKAIYKGPGSEAGP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P21964 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T81 | Phosphorylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| C145 | S-Nitrosylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| Y244 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S267 | Phosphorylation | Uniprot |
研究背景
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
The N-terminus is blocked.
Cytoplasm.
Cell membrane>Single-pass type II membrane protein>Extracellular side.
Brain, liver, placenta, lymphocytes and erythrocytes.
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.
研究領域
· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.
· Metabolism > Amino acid metabolism > Tyrosine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Dopaminergic synapse.
参考文献
Application: WB Species: mouse Sample: cerebral cortices
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