PDE4C Antibody - #DF3872

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製品: PDE4C Antibody
カタログ: DF3872
タンパク質の説明: Rabbit polyclonal antibody to PDE4C
アプリケーション: WB IHC IF/ICC
Cited expt.: IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Dog
分子量: 80 KD; 80kD(Calculated).
ユニプロット: Q08493
RRID: AB_2836225

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(90%), Bovine(90%), Sheep(90%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
PDE4C Antibody detects endogenous levels of total PDE4C.
RRID:
AB_2836225
引用形式: Affinity Biosciences Cat# DF3872, RRID:AB_2836225.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

5''-cyclic phosphodiesterase 4C; cAMP specific 3' 5' cyclic phosphodiesterase 4C; cAMP-specific 3''; DPDE 1; DPDE1; MGC126222; PDE 21; PDE 4C; PDE21; Pde4c; PDE4C_HUMAN; Phosphodiesterase 4C; Phosphodiesterase 4C cAMP specific; Phosphodiesterase 4C, cAMP specific (phosphodiesterase E1 dunce homolog, Drosophila); Phosphodiesterase E1 dunce homolog; Phosphodiesterase E1 dunce homolog Drosophila; Phosphodiesterase type 4C;

免疫原

免疫原:

A synthesized peptide derived from human PDE4C, corresponding to a region within the internal amino acids.

Uniprot:

Q08493(PDE4C_HUMAN) >>Visit HPA database.

遺伝子(ID):
PDE4C(5143)
発現特異性:
Q08493 PDE4C_HUMAN:

Expressed in various tissues but not in cells of the immune system.

タンパク質配列:
MENLGVGEGAEACSRLSRSRGRHSMTRAPKHLWRQPRRPIRIQQRFYSDPDKSAGCRERDLSPRPELRKSRLSWPVSSCRRFDLENGLSCGRRALDPQSSPGLGRIMQAPVPHSQRRESFLYRSDSDYELSPKAMSRNSSVASDLHGEDMIVTPFAQVLASLRTVRSNVAALARQQCLGAAKQGPVGNPSSSNQLPPAEDTGQKLALETLDELDWCLDQLETLQTRHSVGEMASNKFKRILNRELTHLSETSRSGNQVSEYISRTFLDQQTEVELPKVTAEEAPQPMSRISGLHGLCHSASLSSATVPRFGVQTDQEEQLAKELEDTNKWGLDVFKVAELSGNRPLTAIIFSIFQERDLLKTFQIPADTLATYLLMLEGHYHANVAYHNSLHAADVAQSTHVLLATPALEAVFTDLEILAALFASAIHDVDHPGVSNQFLINTNSELALMYNDASVLENHHLAVGFKLLQAENCDIFQNLSAKQRLSLRRMVIDMVLATDMSKHMNLLADLKTMVETKKVTSLGVLLLDNYSDRIQVLQNLVHCADLSNPTKPLPLYRQWTDRIMAEFFQQGDRERESGLDISPMCDKHTASVEKSQVGFIDYIAHPLWETWADLVHPDAQDLLDTLEDNREWYQSKIPRSPSDLTNPERDGPDRFQFELTLEEAEEEDEEEEEEGEETALAKEALELPDTELLSPEAGPDPGDLPLDNQRT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Bovine
90
Sheep
90
Dog
90
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

細胞の位置付け:

Cell projection>Cilium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in various tissues but not in cells of the immune system.

タンパク質ファミリー:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

研究領域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Metabolism > Nucleotide metabolism > Purine metabolism.

参考文献

1). Analysis of the effects of M2 macrophage-derived PDE4C on the prognosis, metastasis and immunotherapy benefit of osteosarcoma. Journal of cellular and molecular medicine, 2024 (PubMed: 38774995) [IF=5.3]

Application: IHC    Species: human    Sample:

FIGURE 5 TAM-derived gene PDE4C was associated metastasis in OS patients. (A) Heatmap exhibited the metastasis condition, M2 macrophage, PDE4C expression in OS from TARGET cohort. (B) Metastasis state between OS patients in high M2 macrophage and low M2 macrophage group from TARGET cohort. (C) Metastasis state between OS patients in high PDE4C and low PDE4C group from TARGET cohort. (D) Diagnostic value of M2 macrophage and PDE4C level for predicting metastasis in OS patients from TARGET cohort. (E) Heatmap exhibited the metastasis condition, M2 macrophage, PDE4C expression in OS from research cohort. (F) Metastasis state between OS patients in high M2 macrophage and low M2 macrophage group from research cohort. (G) Metastasis state between OS patients in high PDE4C and low PDE4C group from research cohort. (H) Diagnostic value of M2 macrophage and PDE4C level for predicting metastasis in OS patients from research cohort. (I and J) IHC was performed to detect the protein levels of PDE4C in OS tissues with and without metastasis. (K) Expression relationship between PDE4C mRNA levels and protein levels. *p 
2). PDE4C stabilized by ELAVL1 promotes lymph node metastasis in papillary thyroid cancer. American journal of cancer research, 2026 (PubMed: 41868681) [IF=3.6]

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