BMP8B Antibody - #DF3850

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製品: BMP8B Antibody
カタログ: DF3850
タンパク質の説明: Rabbit polyclonal antibody to BMP8B
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse
予測: Pig
分子量: 45 KD; 45kD(Calculated).
ユニプロット: P34820
RRID: AB_2836207

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MS Report
HPLC Report
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%)
クローナリティ:
Polyclonal
特異性:
BMP8B Antibody detects endogenous levels of total BMP8B.
RRID:
AB_2836207
引用形式: Affinity Biosciences Cat# DF3850, RRID:AB_2836207.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BMP-8; BMP-8B; BMP8; Bmp8b; BMP8B_HUMAN; bone morphogenetic protein 8 (osteogenic protein 2); Bone morphogenetic protein 8; Bone morphogenetic protein 8B; dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2)); OP-2; OP2; Osteogenic protein 2;

免疫原

免疫原:

A synthesized peptide derived from human BMP8B, corresponding to a region within the internal amino acids.

Uniprot:

P34820(BMP8B_HUMAN) >>Visit HPA database.

遺伝子(ID):
BMP8B(656)
タンパク質配列:
MTALPGPLWLLGLALCALGGGGPGLRPPPGCPQRRLGARERRDVQREILAVLGLPGRPRPRAPPAASRLPASAPLFMLDLYHAMAGDDDEDGAPAERRLGRADLVMSFVNMVERDRALGHQEPHWKEFRFDLTQIPAGEAVTAAEFRIYKVPSIHLLNRTLHVSMFQVVQEQSNRESDLFFLDLQTLRAGDEGWLVLDVTAASDCWLLKRHKDLGLRLYVETEDGHSVDPGLAGLLGQRAPRSQQPFVVTFFRASPSPIRTPRAVRPLRRRQPKKSNELPQANRLPGIFDDVHGSHGRQVCRRHELYVSFQDLGWLDWVIAPQGYSAYYCEGECSFPLDSCMNATNHAILQSLVHLMMPDAVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis (By similarity).

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the TGF-beta family.

研究領域

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

参考文献

1). WDR4-mediate tRNA m7G modification to promote mitophagy and browning of white adipose tissue for ameliorating obesity in male mice. Adipocyte, 2025 (PubMed: 41292047) [IF=3.5]

Application: WB    Species: Mouse    Sample:

Figure 6. WDR4 modulates BMP8B translation via tRNA m7G modification. (a) a volcano plot illustrating the mRNAs with differential te between WDR4-mutant and wild-type 3T3-L1 adipocytes. (b) a heat map of the mRNAs with differential translation efficiency between WDR4-mutant and wild-type 3T3-L1 adipocytes. (c) frequencies of m7G-related codons in TE-increased genes (TE-up), TE-decreased genes (TE-down), and other genes (others) in WDR4-overexpressing 3T3-L1 adipocytes. (d) KEGG enrichment of TE-decreased genes. (e) frequency analysis of the m7G-modified tRnas decoded codons in 3 mRNAs enriched in TGF-β signaling pathway. (f) western blot analysis of TGIF2, amh, and BMP8B expression in WDR4-overexpressing 3T3-L1 adipocytes. (g) the distribution of codons AAG and TGC on the BMP8B gene.

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