MLANA Antibody - #AF0204

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製品: MLANA Antibody
カタログ: AF0204
タンパク質の説明: Rabbit polyclonal antibody to MLANA
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse
分子量: 15kDa; 13kD(Calculated).
ユニプロット: Q16655
RRID: AB_2833288

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
MLANA Antibody detects endogenous levels of total MLANA.
RRID:
AB_2833288
引用形式: Affinity Biosciences Cat# AF0204, RRID:AB_2833288.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Antigen LB39 AA; Antigen LB39-AA; Antigen SK29 AA; Antigen SK29-AA; MAR1_HUMAN; MART 1; MART-1; MART1; Melan A; Melan A protein; Melanoma antigen recognized by T cells 1; Melanoma antigen recognized by T-cells 1; MLAN A; MLANA; OTTHUMP00000021036; OTTHUMP00000021037; OTTHUMP00000021038; Protein Melan-A;

免疫原

免疫原:

A synthesized peptide derived from human MLANA, corresponding to a region within the internal amino acids.

Uniprot:

Q16655(MAR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
MLANA(2315)
発現特異性:
Q16655 MAR1_HUMAN:

Expression is restricted to melanoma and melanocyte cell lines and retina.

タンパク質の説明:
MLANA Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein SILV/PMEL17, which is critical to the formation of stage II melanosomes. Interacts with SILV/PMEL17.
タンパク質配列:
MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

研究背景

機能:

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

PTMs:

Acylated.

細胞の位置付け:

Endoplasmic reticulum membrane>Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus>trans-Golgi network membrane. Melanosome.
Note: Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expression is restricted to melanoma and melanocyte cell lines and retina.

参考文献

1). The HIF1α-PDGFD-PDGFRα axis controls glioblastoma growth at normoxia/mild-hypoxia and confers sensitivity to targeted therapy by echinomycin. Journal of experimental & clinical cancer research : CR, 2021 (PubMed: 34470658) [IF=11.3]
2). Biomimetic polydopamine loaded with janus kinase inhibitor for synergistic vitiligo therapy via hydrogel microneedles. Journal of nanobiotechnology, 2025 (PubMed: 39885576) [IF=10.2]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 7 PDA-JAKi MN improved hair follicle growth in vitiligo mice. (a) Dermal thickness in the dissected mice skin after various treatments. (b) The quantitative analysis of dermal thickness at different groups. (c) MelanA staining after different treatments. Red fields represent hair follicles. (d) The quantitative analysis of the number of MelanA positive cells at different groups. (e) Masson-Fontana staining images of skin and (f) the quantitative analysis of melanin in hair follicles at different groups. (g) Masson-Fontana staining images of epidermal melanin and (h) the quantitative analysis of melanin epidermis at different groups. Red shape indicates melanin. Mean ± SD, n = 3, *P 

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