LYPLA1 Antibody - #DF3737

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製品: LYPLA1 Antibody
カタログ: DF3737
タンパク質の説明: Rabbit polyclonal antibody to LYPLA1
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 25 KD; 25kD(Calculated).
ユニプロット: O75608
RRID: AB_2836101

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Zebrafish(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
LYPLA1 Antibody detects endogenous levels of total LYPLA1.
RRID:
AB_2836101
引用形式: Affinity Biosciences Cat# DF3737, RRID:AB_2836101.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Acyl protein thioesterase 1; Acyl-protein thioesterase 1; APT 1; APT-1; APT1; hAPT1; LPL-I; LPL1; LYPA1_HUMAN; LYPLA 1; LYPLA1; Lysophospholipase 1; Lysophospholipase I; Lysophospholipid specific lysophospholipase; LYSOPLA; LysoPLA I;

免疫原

免疫原:
Uniprot:

O75608(LYPA1_HUMAN) >>Visit HPA database.

遺伝子(ID):
LYPLA1(10434)
タンパク質配列:
MCGNNMSTPLPAIVPAARKATAAVIFLHGLGDTGHGWAEAFAGIRSSHIKYICPHAPVRPVTLNMNVAMPSWFDIIGLSPDSQEDESGIKQAAENIKALIDQEVKNGIPSNRIILGGFSQGGALSLYTALTTQQKLAGVTALSCWLPLRASFPQGPIGGANRDISILQCHGDCDPLVPLMFGSLTVEKLKTLVNPANVTFKTYEGMMHSSCQQEMMDVKQFIDKLLPPID

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
91
Horse
91
Dog
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75608 基板として

Site PTM Type Enzyme
K97 Ubiquitination
K105 Ubiquitination
K190 Ubiquitination
S209 Phosphorylation
S210 Phosphorylation
C211 S-Nitrosylation
K224 Ubiquitination

研究背景

機能:

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer.

タンパク質ファミリー:

Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.

研究領域

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

参考文献

1). Renal lysophospholipase A1 contributes to Enterococcus faecalis-induced hypertension by enhancing sodium reabsorption. iScience (PubMed: 36419851) [IF=5.8]

Application: WB    Species: Mouse    Sample: renal medulla

Figure 1 E. faecalis treatment increased blood pressure and renal LYPLA1 expression in mice (A) Systolic blood pressure (SBP, n = 5 per group) and diastolic blood pressure (DBP, n = 5 per group). (B) Serum Ang II levels (n = 5 per group). (C) Representative immunoblots of LYPLA1 and summarized intensities of blots in the renal medulla (n = 5 per group). (D) mRNA levels of LYPLA1 in the renal medulla (n = 5 per group). (E) Urinary LYPLA1 content (n = 5 per group). (F) Representative immunochemistry staining of LYPLA1 in the kidney and summarized intensities of positive staining (n = 5 per group, bar = 100 μm). Data are presented as mean ± SE. ∗p < 0.05, ∗∗p < 0.01; ns, no significance.

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