ACOT8 Antibody - #DF3736
| 製品: | ACOT8 Antibody |
| カタログ: | DF3736 |
| タンパク質の説明: | Rabbit polyclonal antibody to ACOT8 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 36 KD; 36kD(Calculated). |
| ユニプロット: | O14734 |
| RRID: | AB_2836100 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3736, RRID:AB_2836100.
折りたたみ／展開
ACTEIII; Acot8; ACOT8_HUMAN; Acyl CoA thioesterase 8; Acyl-CoA thioesterase 8; Acyl-coenzyme A thioesterase 8; Choloyl coenzyme A thioesterase; Choloyl-coenzyme A thioesterase; hACTE III; hACTE-III; hACTEIII; HIV Nef associated acyl CoA thioesterase; HIV-Nef-associated acyl-CoA thioesterase; HNAACTE; hTE; Long chain fatty acyl CoA hydrolase; Palmitoyl CoA hydrolase; Peroxisomal acyl CoA thioesterase 1; Peroxisomal acyl coenzyme A thioester hydrolase 1; Peroxisomal acyl-coenzyme A thioester hydrolase 1; Peroxisomal long chain acyl CoA thioesterase 1; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE 1; PTE 2; PTE-1; PTE-2; PTE1; PTE2; Thioesterase II;
免疫原
Detected in a T-cell line (at protein level). Ubiquitous (PubMed:9153233, PubMed:9299485).
- O14734 ACOT8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSPQAPEDGQGCGDRGDPPGDLRSVLVTTVLNLEPLDEDLFRGRHYWVPAKRLFGGQIVGQALVAAAKSVSEDVHVHSLHCYFVRAGDPKLPVLYQVERTRTGSSFSVRSVKAVQHGKPIFICQASFQQAQPSPMQHQFSMPTVPPPEELLDCETLIDQYLRDPNLQKRYPLALNRIAAQEVPIEIKPVNPSPLSQLQRMEPKQMFWVRARGYIGEGDMKMHCCVAAYISDYAFLGTALLPHQWQHKVHFMVSLDHSMWFHAPFRADHWMLYECESPWAGGSRGLVHGRLWRQDGVLAVTCAQEGVIRVKPQVSESKL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14734 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K91 | Acetylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K311 | Acetylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| K318 | Acetylation | Uniprot |
研究背景
Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A thioesterase 8/ACOT8 display no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains. Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity). ACOT8 is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity). It is involved in the metabolic regulation of peroxisome proliferation.
(Microbial infection) May mediate Nef-induced down-regulation of CD4 cell-surface expression.
Peroxisome matrix.
Note: Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded.
Detected in a T-cell line (at protein level). Ubiquitous.
Homodimer (By similarity).
(Microbial infection) Interacts with human immunodeficiency virus (HIV-1) Nef (via middle region); this interaction enhances ACOT8 Acyl-CoA thioesterase activity and occurs in a Nef myristoylation-independent manner. According to a second report, the interaction with HIV-1 Nef occurs in a Nef myristoylation-independent manner but does not enhance ACOT8 Acyl-CoA thioesterase activity.
Belongs to the C/M/P thioester hydrolase family.
研究領域
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
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