製品: ACHE Antibody
カタログ: DF3725
タンパク質の説明: Rabbit polyclonal antibody to ACHE
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Xenopus
分子量: 70 KD; 68kD(Calculated).
ユニプロット: P22303
RRID: AB_2836089

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
ACHE Antibody detects endogenous levels of total ACHE.
RRID:
AB_2836089
引用形式: Affinity Biosciences Cat# DF3725, RRID:AB_2836089.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ACEE; ACES_HUMAN; Acetylcholinesterase; AChE; Apoptosis related acetylcholinesterase; ARACHE; N ACHE; N-ACHE; YT; YT blood group;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P22303 ACES_HUMAN:

Isoform H is highly expressed in erythrocytes.

タンパク質配列:
MRPPQCLLHTPSLASPLLLLLLWLLGGGVGAEGREDAELLVTVRGGRLRGIRLKTPGGPVSAFLGIPFAEPPMGPRRFLPPEPKQPWSGVVDATTFQSVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLVWIYGGGFYSGASSLDVYDGRFLVQAERTVLVSMNYRVGAFGFLALPGSREAPGNVGLLDQRLALQWVQENVAAFGGDPTSVTLFGESAGAASVGMHLLSPPSRGLFHRAVLQSGAPNGPWATVGMGEARRRATQLAHLVGCPPGGTGGNDTELVACLRTRPAQVLVNHEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFHGLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAEFLAGVRVGVPQVSDLAAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLAAQGARVYAYVFEHRASTLSWPLWMGVPHGYEIEFIFGIPLDPSRNYTAEEKIFAQRLMRYWANFARTGDPNEPRDPKAPQWPPYTAGAQQYVSLDLRPLEVRRGLRAQACAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQDRCSDL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
88
Zebrafish
88
Chicken
63
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22303 基板として

Site PTM Type Enzyme
Y164 Phosphorylation
N296 N-Glycosylation
N381 N-Glycosylation
N495 N-Glycosylation
T517 Phosphorylation

研究背景

機能:

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.

細胞の位置付け:

Cell junction>Synapse. Secreted. Cell membrane>Peripheral membrane protein.

Nucleus.
Note: Only observed in apoptotic nuclei.

Cell membrane>Lipid-anchor>Extracellular side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform H is highly expressed in erythrocytes.

サブユニット構造:

Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers (By similarity). Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers. Isoform R may be monomeric.

タンパク質ファミリー:

Belongs to the type-B carboxylesterase/lipase family.

研究領域

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Organismal Systems > Nervous system > Cholinergic synapse.

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