ABHD7 Antibody - #DF3721

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製品: ABHD7 Antibody
カタログ: DF3721
タンパク質の説明: Rabbit polyclonal antibody to ABHD7
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 42 KD(Observed); 42kD(Calculated).
ユニプロット: Q8IUS5
RRID: AB_2836085

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#DF3721-BP

ABHD7 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(92%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
クローナリティ:
Polyclonal
特異性:
ABHD7 Antibody detects endogenous levels of total ABHD7.
RRID:
AB_2836085
引用形式: Affinity Biosciences Cat# DF3721, RRID:AB_2836085.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ABHD7; Abhydrolase domain containing 7; Abhydrolase domain-containing protein 7; Ephx4; EPHX4_HUMAN; EPHXRP; Epoxide hydrolase 4; Epoxide hydrolase-related protein;

免疫原

免疫原:

A synthesized peptide derived from human ABHD7, corresponding to a region within the internal amino acids.

Uniprot:

Q8IUS5(EPHX4_HUMAN) >>Visit HPA database.

遺伝子(ID):
EPHX4(253152)
タンパク質配列:
MARLRDCLPRLMLTLRSLLFWSLVYCYCGLCASIHLLKLLWSLGKGPAQTFRRPAREHPPACLSDPSLGTHCYVRIKDSGLRFHYVAAGERGKPLMLLLHGFPEFWYSWRYQLREFKSEYRVVALDLRGYGETDAPIHRQNYKLDCLITDIKDILDSLGYSKCVLIGHDWGGMIAWLIAICYPEMVMKLIVINFPHPNVFTEYILRHPAQLLKSSYYYFFQIPWFPEFMFSINDFKVLKHLFTSHSTGIGRKGCQLTTEDLEAYIYVFSQPGALSGPINHYRNIFSCLPLKHHMVTTPTLLLWGENDAFMEVEMAEVTKIYVKNYFRLTILSEASHWLQQDQPDIVNKLIWTFLKEETRKKD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Sheep
92
Bovine
83
Zebrafish
70
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

細胞の位置付け:

Membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.

参考文献

1). ABHD7-mediated depalmitoylation of lamin A promotes myoblast differentiation. Cell reports, 2024 (PubMed: 38308845) [IF=7.5]

Application: WB    Species: Human    Sample: HEK293T cells

Figure 3ABHD7 is the depalmitoylase of lamin A (A) Exogenous ABHD5, -7, -17A, -17C, and -18 interact with lamin A. Myc-tagged lamin A was co-expressed with FLAG-tagged ABHDs, PPTs, or APTs in HEK293T cells, followed by immunoprecipitation for western blotting. (B) ABHD7 interacts with endogenous lamin A. Endogenous lamin A was detected by western blotting in HEK293T cells expressing FLAG-tagged ABHDs. (C) Overexpression of ABHD7 reduces palmitoylation of lamin A in HEK293T cells. The palmitoylation of lamin A was detected by ABE assay in HEK293T cells expressing FLAG-tagged lamin A and Myc-tagged ABHD7. “Ratio,” biotin/FLAG. (D) Knocking down ABHD7 increases exogenous palmitoylation of lamin A. The palmitoylation of FLAG-tagged lamin A was detected in ABHD7-knockdown HEK293T cells through ABE assay. “Ratio,” biotin/FLAG. (E) Endogenous palmitoylation of lamin A is increased in C2C12 cells with Abhd7 knockdown. The palmitoylation of lamin A was determined through ABE assay. “Ratio,” biotin/lamin A. (F) Immunofluorescence staining shows co-localization of Abhd7 (green) and lamin A/C (red) at the nucleus region. Scale bar: 50 μm. Data in (A)–(F) are representative of three biologically independent experiments.

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