RPL5 Antibody - #DF3712
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3712, RRID:AB_2836076.
折りたたみ／展開
60S ribosomal protein L5; DBA6; L5; MSTP030; PPP1R135; protein phosphatase 1, regulatory subunit 135; Ribosomal protein L5; RL5; RL5_HUMAN; RP L5; RPL 5; RPL5; U21 RNA; U21RNA;
免疫原
A synthesized peptide derived from human RPL5, corresponding to a region within C-terminal amino acids.
- P46777 RL5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P46777 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| G2 | Acetylation | Uniprot | |
| K5 | Acetylation | Uniprot | |
| K5 | Methylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K27 | Acetylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K43 | Acetylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| Y66 | Phosphorylation | Uniprot | |
| C76 | S-Nitrosylation | Uniprot | |
| C100 | S-Nitrosylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K164 | Methylation | Uniprot | |
| K164 | Sumoylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| K178 | Acetylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| K188 | Sumoylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Sumoylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| R209 | Methylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K220 | Sumoylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K221 | Acetylation | Uniprot | |
| K221 | Sumoylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K228 | Methylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| K241 | Acetylation | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| R248 | Methylation | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| K276 | Acetylation | Uniprot | |
| K283 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S286 | Phosphorylation | Uniprot |
研究背景
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53.
Cytoplasm. Nucleus>Nucleolus.
Component of the large ribosomal subunit (LSU). Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11. Interacts with isoform 1 of NVL in an ATP-dependent manner. Interacts with RRP1B.
Belongs to the universal ribosomal protein uL18 family.
研究領域
· Genetic Information Processing > Translation > Ribosome.
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