PARP2 Antibody - #DF3625
| 製品: | PARP2 Antibody |
| カタログ: | DF3625 |
| タンパク質の説明: | Rabbit polyclonal antibody to PARP2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 75 KD; 66kD(Calculated). |
| ユニプロット: | Q9UGN5 |
| RRID: | AB_2835997 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3625, RRID:AB_2835997.
折りたたみ／展開
ADP ribosyltransferase like 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADPRT 2; ADPRT-2; ADPRT2; ADPRTL 2; ADPRTL 3; ADPRTL2; ADPRTL3; ARTD2; hPARP 2; hPARP-2; hPARP2; NAD(+) ADP ribosyltransferase 2; NAD(+) ADP-ribosyltransferase 2; pADPRT 2; pADPRT-2; pADPRT2; PARP 2; PARP-2; PARP2; PARP2_HUMAN; Poly (ADP ribose) polymerase family member 2; Poly (ADP ribosyl) transferase like 2; poly (ADP-ribose) polymerase 2; Poly [ADP ribose] synthetase 2; Poly [ADP-ribose] polymerase 2; Poly(ADP ribose) synthetase; Poly[ADP-ribose] synthase 2;
免疫原
Widely expressed, mainly in actively dividing tissues (PubMed:10364231). The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus (PubMed:10364231).
- Q9UGN5 PARP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAARRRRSTGGGRARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDGMPGRSWASKRVSESVKALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGRVGKMGQHSLVACSGNLNKAKEIFQKKFLDKTKNNWEDREKFEKVPGKYDMLQMDYATNTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UGN5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| K516 | Ubiquitination | Uniprot |
研究背景
Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex.
Poly-ADP-ribosylated by PARP1.
Acetylation reduces DNA binding and enzymatic activity.
Nucleus.
Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus.
Component of a base excision repair (BER) complex, containing at least XRCC1, PARP1, POLB and LRIG3 (By similarity). Homo- and heterodimer with PARP1. Interacts weakly (via the PARP catalytic domain) with HPF1.
研究領域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Genetic Information Processing > Replication and repair > Base excision repair.
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