Cytochrome P450 4Z1 Antibody - #DF3590

(4)   (1)  
製品: Cytochrome P450 4Z1 Antibody
カタログ: DF3590
タンパク質の説明: Rabbit polyclonal antibody to Cytochrome P450 4Z1
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse, Rat
分子量: 59 KD(Observed); 59kD(Calculated).
ユニプロット: Q86W10
RRID: AB_2835962

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Cytochrome P450 4Z1 Antibody detects endogenous levels of total Cytochrome P450 4Z1.
RRID:
AB_2835962
引用形式: Affinity Biosciences Cat# DF3590, RRID:AB_2835962.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CP4Z1_HUMAN; CYP4A20; CYP4Z1; CYPIVZ1; Cytochrome P450 4Z1; Cytochrome P450 family 4 subfamily Z polypeptide 1;

免疫原

免疫原:

A synthesized peptide derived from human Cytochrome P450 4Z1, corresponding to a region within N-terminal amino acids.

Uniprot:

Q86W10(CP4Z1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CYP4Z1(199974)
発現特異性:
Q86W10 CP4Z1_HUMAN:

Preferentially detected in breast carcinoma tissue and mammary gland, whereas only marginal expression is found in all other tested tissues.

タンパク質配列:
MEPSWLQELMAHPFLLLILLCMSLLLFQVIRLYQRRRWMIRALHLFPAPPAHWFYGHKEFYPVKEFEVYHKLMEKYPCAVPLWVGPFTMFFSVHDPDYAKILLKRQDPKSAVSHKILESWVGRGLVTLDGSKWKKHRQIVKPGFNISILKIFITMMSESVRMMLNKWEEHIAQNSRLELFQHVSLMTLDSIMKCAFSHQGSIQLDSTLDSYLKAVFNLSKISNQRMNNFLHHNDLVFKFSSQGQIFSKFNQELHQFTEKVIQDRKESLKDKLKQDTTQKRRWDFLDILLSAKSENTKDFSEADLQAEVKTFMFAGHDTTSSAISWILYCLAKYPEHQQRCRDEIRELLGDGSSITWEHLSQMPYTTMCIKECLRLYAPVVNISRLLDKPITFPDGRSLPAGITVFINIWALHHNPYFWEDPQVFNPLRFSRENSEKIHPYAFIPFSAGLRNCIGQHFAIIECKVAVALTLLRFKLAPDHSRPPQPVRQVVLKSKNGIHVFAKKVC

研究背景

細胞の位置付け:

Endoplasmic reticulum membrane>Single-pass type II membrane protein. Microsome membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Preferentially detected in breast carcinoma tissue and mammary gland, whereas only marginal expression is found in all other tested tissues.

タンパク質ファミリー:

Belongs to the cytochrome P450 family.

参考文献

1). Plasma membrane localization of CYP4Z1 and CYP19A1 and the detection of anti-CYP19A1 autoantibodies in humans. International Immunopharmacology, 2019 (PubMed: 31082724) [IF=4.8]

Application: IF/ICC    Species: human    Sample: MCF-7 or MCF-10A cells

Fig. 2.| Detection of CYP4Z1 on the outer surface of non-permeabilized MCF-7 or MCF-10A cells by rabbit anti-CYP4Z1 IgG and goat anti-rabbit IgG conjugated to Alexa fluor 488. The non-permeabilized cells were probed with antibodies and stained with DAPI and Deep Red plasma membrane stain as indicated. Magnification is×1,000 for all images
2). Concomitant occurence of multiple autoantibodies against human cytochromes P450. International Immunopharmacology, 2021 (PubMed: 34464888) [IF=4.8]
3). Involvement of WNT2 in trophoblast cell behavior in preeclampsia development. CELL CYCLE, 2020 (PubMed: 32779546) [IF=3.4]
4). Upregulation of estrogen receptor alpha (ERα) expression in transgenic mice expressing human CYP4Z1. Breast Cancer Research and Treatment, 2021 (PubMed: 34725776) [IF=3.0]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.