| 製品: | MAEA Antibody |
| カタログ: | DF3332 |
| タンパク質の説明: | Rabbit polyclonal antibody to MAEA |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 45 KD(Observed); 45kD(Calculated). |
| ユニプロット: | Q7L5Y9 |
| RRID: | AB_2835699 |
Control Products
製品説明
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3332, RRID:AB_2835699.
折りたたみ／展開
1110030D19Rik; Cell proliferation inducing gene 5 protein; Cell proliferation-inducing gene 5 protein; EMLP; EMP; Erythroblast macrophage protein; GID complex subunit 9 FYV10 homolog; GID9; HLC 10; HLC-10; HLC10; Human lung cancer oncogene 10 protein; Lung cancer related protein 10; Macrophage erythroblast attacher; MAEA; MAEA_HUMAN; MGC93683; PIG5; Proliferation inducing gene 5;
免疫原
A synthesized peptide derived from human MAEA, corresponding to a region within the internal amino acids.
- Q7L5Y9 MAEA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVQESAAQLSMTLKVQEYPTLKVPYETLNKRFRAAQKNIDRETSHVTMVVAELEKTLSGCPAVDSVVSLLDGVVEKLSVLKRKAVESIQAEDESAKLCKRRIEHLKEHSSDQPAAASVWKRKRMDRMMVEHLLRCGYYNTAVKLARQSGIEDLVNIEMFLTAKEVEESLERRETATCLAWCHDNKSRLRKMKSCLEFSLRIQEFIELIRQNKRLDAVRHARKHFSQAEGSQLDEVRQAMGMLAFPPDTHISPYKDLLDPARWRMLIQQFRYDNYRLHQLGNNSVFTLTLQAGLSAIKTPQCYKEDGSSKSPDCPVCSRSLNKLAQPLPMAHCANSRLVCKISGDVMNENNPPMMLPNGYVYGYNSLLSIRQDDKVVCPRTKEVFHFSQAEKVYIM
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation. The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1. Plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages (By similarity). Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis. Participates in erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages (By similarity). May contribute to nuclear architecture and cells division events (Probable).
Autoubiquitinated as component of the CTLH E3 ubiquitin-protein ligase complex (in vitro).
Cytoplasm. Nucleus>Nucleoplasm. Nucleus matrix. Cell membrane. Cytoplasm>Cytoskeleton.
Note: Detected in a nuclear, speckled-like pattern (PubMed:16510120). Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis (PubMed:16510120). Present in cytoplasm, nuclear matrix and at the cell surface in macrophages; predominantly nuclear in immature macrophages and predominantly detected at the cell surface in mature macrophages. Colocalizes with F-actin in macrophages (By similarity).
Detected at macrophage membranes (at protein level). Ubiquitous.
The expected RING-type zinc finger domain is highly divergent and most of the expected Cys residues are not conserved (Probable). Still, the protein is required for CTLH complex E3 ubiquitin-protein transferase activity (PubMed:29911972). In addition, the conserved Cys-314 in this highly divergent region is required for ubiquitination by the yeast GID complex, suggesting a direct role in catalyzing ubiquitination (Probable).
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