TNFAIP8L2 Antibody - #DF3326

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製品: TNFAIP8L2 Antibody
カタログ: DF3326
タンパク質の説明: Rabbit polyclonal antibody to TNFAIP8L2
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 20 KD; 21kD(Calculated).
ユニプロット: Q6P589
RRID: AB_2835693

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
TNFAIP8L2 Antibody detects endogenous levels of total TNFAIP8L2.
RRID:
AB_2835693
引用形式: Affinity Biosciences Cat# DF3326, RRID:AB_2835693.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AW610835; FLJ23467; Inflammation factor 20; Inflammation factor protein 20; TIPE2; TNF alpha-induced protein 8-like protein 2; TNFAIP8-like protein 2; TNFAIP8L2; TP8L2_HUMAN; Tumor necrosis factor alpha induced protein 8 like 2; Tumor necrosis factor alpha-induced protein 8-like protein 2;

免疫原

免疫原:

A synthesized peptide derived from human TNFAIP8L2, corresponding to a region within the internal amino acids.

Uniprot:

Q6P589(TP8L2_HUMAN) >>Visit HPA database.

遺伝子(ID):
TNFAIP8L2(79626)
タンパク質配列:
MESFSSKSLALQAEKKLLSKMAGRSVAHLFIDETSSEVLDELYRVSKEYTHSRPQAQRVIKDLIKVAIKVAVLHRNGSFGPSELALATRFRQKLRQGAMTALSFGEVDFTFEAAVLAGLLTECRDVLLELVEHHLTPKSHGRIRHVFDHFSDPGLLTALYGPDFTQHLGKICDGLRKLLDEGKL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Rabbit
100
Bovine
91
Sheep
91
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Acts as a negative regulator of innate and adaptive immunity by maintaining immune homeostasis. Negative regulator of Toll-like receptor and T-cell receptor function. Prevents hyperresponsiveness of the immune system and maintains immune homeostasis. Inhibits JUN/AP1 and NF-kappa-B activation. Promotes Fas-induced apoptosis (By similarity).

タンパク質ファミリー:

The central region was initially thought to constitute a DED (death effector) domain. However, 3D-structure data reveal a previously uncharacterized fold that is different from the predicted fold of a DED (death effector) domain. It consists of a large, hydrophobic central cavity that is poised for cofactor binding.

Belongs to the TNFAIP8 family. TNFAIP8L2 subfamily.

参考文献

1). 7-Difluoromethoxy-5, 4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice. International Immunopharmacology, 2021 (PubMed: 33813367) [IF=4.8]

Application: WB    Species: mice    Sample: RAW 264.7 cells

Fig. 8. Effects of DFMG on apoptosis and expression of TIPE2 and TLR4 in LPC-induced RAW 264.7 cells. We used DFMG (0.3 μmol/L) to preincubate RAW264.7 cells for 0.5 h and then stimulated by LPC (1 μg/mL) for 24 h. (A) The rate of apoptosis was detected by Annexin V-FITC/PI kit. (B) The levels of TIPE2 and TLR4 mRNA were measured by RT-qPCR. (C) The expression of TIPE2 and TLR4 protein were measured by Western blot. n = 3, Data are presented as mean ± SD from three independent experiments. **P < 0.01.
2). Tumor necrosis factor α‑induced protein 8‑like 2 contributes to penehyclidine hydrochloride pretreatment against lipopolysaccharide‑induced acute lung injury in a mouse model. Molecular Medicine Reports, 2021 (PubMed: 34468006) [IF=3.4]
3). Genistein promotes M1 macrophage apoptosis and reduces inflammatory response by disrupting miR-21/TIPE2 pathway. SAUDI PHARMACEUTICAL JOURNAL, 2022 (PubMed: 35903524) [IF=3.0]
4). Expression and regulation of tumor necrosis factor-α-induced protein-8-like 2 is associated with acute lung injury induced by myocardial ischemia reperfusion in diabetic rats. Microvascular Research, 2020 (PubMed: 32333940) [IF=2.9]

Application: WB    Species: rat    Sample: lung

Fig. 5.| Protein expression of Bax, Bcl-2, cleaved caspase 3, cleaved caspase 9, JNK, p-JNK, TIPE2 and nuclear NF-κB p65 in lung tissues collected after MIR or sham procedure. (a) Western blotting analysis of protein expression.

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