FHL2 Recombinant Rabbit mAb - #BF4532

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製品: FHL2 Recombinant Rabbit mAb
カタログ: BF4532
タンパク質の説明: Rabbit monoclonal antibody to FHL2
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
分子量: 32 kDa(Observed); 32kD(Calculated).
ユニプロット: Q14192

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製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
クローナリティ:
Monoclonal [ReFirm23982]
特異性:
FHL2 Recombinant Rabbit mAb detects endogenous levels of FHL2.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AAG 11; AAG11; Aging associated gene 11; Down regulated in rhabdomyosarcoma LIM protein; Downregulated in rhabdomyosarcoma LIM protein; DRAL; FHL 2; FHL-2; Fhl2; FHL2 protein; FHL2_HUMAN; Four and a half LIM domain protein 2; Four and a half LIM domains 2; Four and a half LIM domains protein 2; KIAA0990; LIM domain protein DRAL; Skeletal muscle LIM protein 3; Skeletal muscle LIM-protein 3; SLIM 3; SLIM-3; SLIM3;

免疫原

免疫原:

A synthetic peptide from human FHL2

Uniprot:

Q14192(FHL2_HUMAN) >>Visit HPA database.

遺伝子(ID):
FHL2(2274)
発現特異性:
Q14192 FHL2_HUMAN:

Expressed in skeletal muscle and heart.

タンパク質の説明:
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved,tandemly arranged,zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also,this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified.
タンパク質配列:
MTERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI

研究背景

機能:

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes.

細胞の位置付け:

Cytoplasm. Nucleus. Cytoplasm>Myofibril>Sarcomere>Z line.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in skeletal muscle and heart.

タンパク質ファミリー:

The third LIM zinc-binding mediates interaction with E4F1.

研究領域

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

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