ARMET Recombinant Rabbit mAb - #BF4491

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製品: ARMET Recombinant Rabbit mAb
カタログ: BF4491
タンパク質の説明: Rabbit monoclonal antibody to ARMET
アプリケーション: WB IHC IF/ICC
反応性: Human
分子量: 18 kDa(Observed); 21kD(Calculated).
ユニプロット: P55145

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製品説明

ソース:
Rabbit IgG
アプリケーション:
IHC 1:50-1:200, WB 1:1000, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [ReFirm23941]
特異性:
ARMET Recombinant Rabbit mAb detects endogenous levels of ARMET.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Arginine rich mutated in early stage tumors; Arginine rich protein; Arginine-rich protein; ARMET; ARMET protein; ARP; Manf; MANF_HUMAN; Mesencephalic astrocyte derived neurotrophic factor; Mesencephalic astrocyte-derived neurotrophic factor; MGC142148; MGC142150; Protein ARMET;

免疫原

免疫原:

A synthetic peptide from human ARMET

Uniprot:

P55145(MANF_HUMAN) >>Visit HPA database.

遺伝子(ID):
MANF(7873)
タンパク質の説明:
The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi,and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region,including a specific mutation that changes an ATG start codon to AGG,have been reported in a variety of solid tumors,however,these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.
タンパク質配列:
MRRMWATQGLAVALALSVLPGSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

研究背景

機能:

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions.

PTMs:

May contain sialic acid residues.

細胞の位置付け:

Secreted. Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen.
Note: Retained in the endoplasmic reticulum (ER), and sarcoplasmic reticulum (SR) under normal conditions (PubMed:22637475). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475, PubMed:29497057).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

The N-terminal region may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response.

The N-terminal region may be required for lipid sulfatide binding.

Belongs to the ARMET family.

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