IRF8 Recombinant Rabbit mAb - #BF4462

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製品: IRF8 Recombinant Rabbit mAb
カタログ: BF4462
タンパク質の説明: Rabbit monoclonal antibody to IRF8
アプリケーション: WB IHC
反応性: Human
分子量: 48 kDa(Observed); 48kD(Calculated).
ユニプロット: Q02556

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製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [ReFirm23912]
特異性:
IRF8 Recombinant Rabbit mAb detects endogenous levels of IRF8.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

H ICSBP; H-ICSBP; HGNC:5358; HICSBP; ICSBP 1; ICSBP; ICSBP1; Interferon consensus sequence binding protein 1; Interferon consensus sequence binding protein; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF 8; IRF-8; Irf8; IRF8_HUMAN; MYLS;

免疫原

免疫原:

A synthetic peptide from human IRF8

Uniprot:

Q02556(IRF8_HUMAN) >>Visit HPA database.

遺伝子(ID):
IRF8(3394)
発現特異性:
Q02556 IRF8_HUMAN:

Predominantly expressed in lymphoid tissues.

タンパク質の説明:
Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs,namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection.
タンパク質配列:
MCDRNGGRRLRQWLIEQIDSSMYPGLIWENEEKSMFRIPWKHAGKQDYNQEVDASIFKAWAVFKGKFKEGDKAEPATWKTRLRCALNKSPDFEEVTDRSQLDISEPYKVYRIVPEEEQKCKLGVATAGCVNEVTEMECGRSEIDELIKEPSVDDYMGMIKRSPSPPEACRSQLLPDWWAQQPSTGVPLVTGYTTYDAHHSAFSQMVISFYYGGKLVGQATTTCPEGCRLSLSQPGLPGTKLYGPEGLELVRFPPADAIPSERQRQVTRKLFGHLERGVLLHSSRQGVFVKRLCQGRVFCSGNAVVCKGRPNKLERDEVVQVFDTSQFFRELQQFYNSQGRLPDGRVVLCFGEEFPDMAPLRSKLILVQIEQLYVRQLAEEAGKSCGAGSVMQAPEEPPPDQVFRMFPDICASHQRSFFRENQQITV

研究背景

機能:

Plays a role as a transcriptional activator or repressor. Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Positively regulates macroautophagy in dendritic cells.

PTMs:

Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes (By similarity). Ubiquitination leads to its degradation by the proteasome.

Sumoylated with SUMO3. Desumoylated by SENP1.

細胞の位置付け:

Nucleus. Cytoplasm.
Note: In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN-gamma induction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Predominantly expressed in lymphoid tissues.

タンパク質ファミリー:

Belongs to the IRF family.

研究領域

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

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