NUDT1 Recombinant Rabbit mAb - #BF4453

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製品: NUDT1 Recombinant Rabbit mAb
カタログ: BF4453
タンパク質の説明: Rabbit monoclonal antibody to NUDT1
アプリケーション: WB IHC IF/ICC
反応性: Human
分子量: 18-24kD(Observed); 23kD(Calculated).
ユニプロット: P36639

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製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000-1:5000, IHC 1:50, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [ReFirm23903]
特異性:
NUDT1 Recombinant Rabbit mAb detects endogenous levels of NUDT1.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

2-hydroxy-dATP diphosphatase; 7 8 dihydro 8 oxoguanine triphosphatase; 7; 8 oxo 7 8 dihydrodeoxyguanosine triphosphatase; 8 oxo 7 8 dihydroguanosine triphosphatase; 8 oxo dGTPase; 8-dihydro-8-oxoguanine triphosphatase; 8-oxo-dGTPase; 8ODP_HUMAN; MTH 1; MTH1; MutT human homolog 1; Nucleoside diphosphate linked moiety X motif 1; Nucleoside diphosphate linked moiety X type motif 1; Nucleoside diphosphate-linked moiety X motif 1; Nudix (nucleoside diphosphate linked moiety X) type motif 1; Nudix hydrolase 1; Nudix motif 1; Nudix type motif 1; NUDT 1; Nudt1;

免疫原

免疫原:

A synthetic peptide from human NUDT1

Uniprot:

P36639(8ODP_HUMAN) >>Visit HPA database.

遺伝子(ID):
NUDT1(4521)
発現特異性:
P36639 8ODP_HUMAN:

Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes.

タンパク質の説明:
Misincorporation of oxidized nucleoside triphosphates into DNA,RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates,such as 8-oxo-dGTP,8-oxo-dATP,2-hydroxy-dATP,and 2-hydroxy rATP,to monophosphates,thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm,with some in the mitochondria,suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants,some of which encode distinct isoforms,have been identified. Additional variants have been observed,but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional,longer isoform (p26) has been described.
タンパク質配列:
MYWSNQITRRLGERVQGFMSGISPQQMGEPEGSWSGKNPGTMGASRLYTLVLVLQPQRVLLGMKKRGFGAGRWNGFGGKVQEGETIEDGARRELQEESGLTVDALHKVGQIVFEFVGEPELMDVHVFCTDSIQGTPVESDEMRPCWFQLDQIPFKDMWPDDSYWFPLLLQKKKFHGYFKFQGQDTILDYTLREVDTV

研究背景

機能:

Antimutagenic. Plays a redundant role in sanitizing oxidized nucleotide pools, such as 8-oxo-dGTP pools. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA (By similarity).

PTMs:

The N-terminus is blocked.

細胞の位置付け:

Cytoplasm>Cytosol. Mitochondrion matrix. Nucleus.
Note: Mostly present in cytosol (PubMed:7782328). A minor proportion is mitochondrial (PubMed:7782328). A very small amount of the protein is associated with nuclei (PubMed:7782328). Variant Met-124 has decreased efficiency in translocation to mitochondria (PubMed:16607562).

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes.

タンパク質ファミリー:

Belongs to the Nudix hydrolase family.

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