製品: SFRS17A Antibody
カタログ: DF3235
タンパク質の説明: Rabbit polyclonal antibody to SFRS17A
アプリケーション: WB IF/ICC
Cited expt.:
反応性: Human, Mouse
予測: Horse, Dog, Chicken
分子量: 81 KD; 81kD(Calculated).
ユニプロット: Q02040
RRID: AB_2835615

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Horse(86%), Dog(86%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
SFRS17A Antibody detects endogenous levels of total SFRS17A.
RRID:
AB_2835615
引用形式: Affinity Biosciences Cat# DF3235, RRID:AB_2835615.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

721P; A-kinase anchor protein 17A; AK17A_HUMAN; AKAP-17A; AKAP17A; arginine/serine-rich 17A; B lymphocyte antigen; B lymphocyte surface antigen; B-lymphocyte antigen; CCDC133; PRKA17A; Protein kinase A-anchoring protein 17A; Protein XE7; Pseudoautosomal gene XE7; SFRS 17A; Splicing factor; Splicing factor arginine/serine rich 17A; XE 7; XE7;

免疫原

免疫原:

A synthesized peptide derived from human SFRS17A, corresponding to a region within C-terminal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q02040 AK17A_HUMAN:

Widely expressed. Found in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Expressed in activated B-cells and placenta. Expressed in all cell lines tested including Jurkat-TAg, U-937 and HEK293 cells.

タンパク質配列:
MAAATIVHDTSEAVELCPAYGLYLKPITKMTISVALPQLKQPGKSISNWEVMERLKGMVQNHQFSTLRISKSTMDFIRFEGEVENKSLVKSFLACLDGKTIKLSGFSDILKVRAAEFKIDFPTRHDWDSFFRDAKDMNETLPGERPDTIHLEGLPCKWFALKESGSEKPSEDVLVKVFEKFGEIRNVDIPMLDPYREEMTGRNFHTFSFGGHLNFEAYVQYREYMGFIQAMSALRGMKLMYKGEDGKAVACNIKVSFDSTKHLSDASIKKRQLERQKLQELEQQREEQKRREKEAEERQRAEERKQKELEELERERKREEKLRKREQKQRDRELRRNQKKLEKLQAEEQKQLQEKIKLEERKLLLAQRNLQSIRLIAELLSRAKAVKLREQEQKEEKLRLQQQEERRRLQEAELRRVEEEKERALGLQRKERELRERLLSILLSKKPDDSHTHDELGVAHADLLQPVLDILQTVSSGCVSATTLHPLGGQPPAGAPKESPAHPEADGAPKSVNGSVAEEAPCKEVQSSCRVVPEDGSPEKRCPGGVLSCIPDNNQQPKGIPACEQNVSRKDTRSEQDKCNREPSKGRGRATGDGLADRHKRERSRARRASSREDGRPRKERRPHKKHAYKDDSPRRRSTSPDHTRSRRSHSKDRHRRERSRERRGSASRKHSRHRRRSERSRSRSPSRHRSTWNR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
86
Dog
86
Chicken
83
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Splice factor regulating alternative splice site selection for certain mRNA precursors. Mediates regulation of pre-mRNA splicing in a PKA-dependent manner.

細胞の位置付け:

Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Found in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Expressed in activated B-cells and placenta. Expressed in all cell lines tested including Jurkat-TAg, U-937 and HEK293 cells.

タンパク質ファミリー:

RI-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

参考文献

1). Comprehensive bioinformatics analysis reveals the significance of forkhead box family members in pancreatic adenocarcinoma. Aging, 2023 (PubMed: 36622275) [IF=3.9]

Application: IHC    Species: human    Sample:

Figure 7.Validation of the prognostic role of FOXs in PAAD patient tissues. (A) Representative immunohistochemistry (IHC) images (200X) in PAAD tissues with low or high FOXM1, FOXP1, FOXN3, FOXF2, and FOXK1 protein levels. (B) Expression of FOXM1, FOXP1, and FOXN3 was negatively correlated with OS in PAAD patient pathology samples. Expression of FOXF2 and FOXK1 had no significant correlation with OS.

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