PLEC Recombinant Rabbit mAb - #BF4273
製品説明
ソース:
Rabbit IgG
アプリケーション:
WB 1:1000-1:5000, IHC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human, Mouse, Rat
クローナリティ:
Monoclonal [ReFirm23723]
特異性:
PLEC Recombinant Rabbit mAb detects endogenous levels of PLEC.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
EBS1; EBSO; HD1; Hemidesmosomal protein 1; PCN; pleC; PLEC_HUMAN; PLEC1; PLEC1b; Plectin 1; Plectin 1 intermediate filament binding protein 500kDa; Plectin 6; Plectin; Plectin-1; PLTN;
免疫原
免疫原:
A synthetic peptide from human PLEC
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質の説明:
Plectin is a prominent member of an important family of structurally and in part functionally related proteins,termed plakins or cytolinkers,that are capable of interlinking different elements of the cytoskeleton. Plakins,with their multi-domain structure and enormous size,not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape,but also serve as scaffolding platforms for the assembly,positioning,and regulation of signaling complexes (reviewed in PMID: 9701547,11854008,and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans (PMID: 8633055,8698233). Until 2010,this locus was named plectin 1 (symbol PLEC1 in human,Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1,intermediate filament binding 500kDa". These names were superseded by plectin. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294,14559777),revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1,0a,and 0) splice into an alternative first coding exon (1c),and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974),exons 1i,1j and 1h have not been confirmed in human.
タンパク質配列:
- Q15149 PLEC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
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研究背景
機能:
Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.
PTMs:
Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
細胞の位置付け:
Cytoplasm>Cytoskeleton. Cell junction>Hemidesmosome.
組織特異性:
Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
タンパク質ファミリー:
The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N- and the C-terminus can bind integrin beta-4.
Belongs to the plakin or cytolinker family.
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