Glutathione Peroxidase 4 Recombinant Rabbit mAb - #BF4227

WB 1:1000-1:5000, IHC: 1:50-1:200, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Human, Mouse, Rat

Monoclonal [ReFirm23677]

Glutathione Peroxidase 4 Recombinant Rabbit mAb detects endogenous levels of Glutathione Peroxidase 4.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

The protein encoded by this gene belongs to the glutathione peroxidase family,members of which catalyze the reduction of hydrogen peroxide,organic hydroperoxides and lipid hydroperoxides,and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates,which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development,thus,it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase,as well as a structural protein in mature spermatozoa. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein,containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon,which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure,designated the Sec insertion sequence (SECIS) element,that is necessary for the recognition of UGA as a Sec codon,rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. Pseudogenes of this locus have been identified on chromosomes 10 and 17.