alpha 5 Defensin Recombinant Rabbit mAb - #BF4161

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製品: alpha 5 Defensin Recombinant Rabbit mAb
カタログ: BF4161
タンパク質の説明: Rabbit monoclonal antibody to alpha 5 Defensin
アプリケーション: WB IHC
反応性: Human
分子量: 10 kDa(Observed); 10kD(Calculated).
ユニプロット: Q01523

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製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000-1:5000, IHC 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [ReFirm23608]
特異性:
alpha 5 Defensin Recombinant Rabbit mAb detects endogenous levels of alpha 5 Defensin.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DEF5,HD-5

免疫原

免疫原:

A synthetic peptide from human alpha 5 Defensin

Uniprot:

Q01523(DEF5_HUMAN) >>Visit HPA database.

遺伝子(ID):
DEFA5(1670)
発現特異性:
Q01523 DEF5_HUMAN:

Paneth cells of the small intestine (at protein level).

タンパク質の説明:
Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine,respiratory tract,urinary tract,and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene,defensin,alpha 5,is highly expressed in the secretory granules of Paneth cells of the ileum.
タンパク質配列:
MRTIAILAAILLVALQAQAESLQERADEATTQKQSGEDNQDLAISFAGNGLSALRTSGSQARATCYCRTGRCATRESLSGVCEISGRLYRLCCR

研究背景

機能:

Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.

PTMs:

Glycosylated.

細胞の位置付け:

Secreted. Cytoplasmic vesicle>Secretory vesicle.
Note: Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Paneth cells of the small intestine (at protein level).

タンパク質ファミリー:

Belongs to the alpha-defensin family.

Restrictive clause

 

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For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.