ARPC5 Recombinant Rabbit mAb - #BF3973

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製品: ARPC5 Recombinant Rabbit mAb
カタログ: BF3973
タンパク質の説明: Rabbit monoclonal antibody to ARPC5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 16 kDa(Observed); 16kD(Calculated).
ユニプロット: O15511

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製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000-1:5000, IHC 1:50, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
クローナリティ:
Monoclonal [ReFirm23420]
特異性:
ARPC5 Recombinant Rabbit mAb detects endogenous levels of ARPC5.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ARC16,p16-Arc,dJ127C7.3

免疫原

免疫原:

A synthetic peptide from human ARPC5

Uniprot:

O15511(ARPC5_HUMAN) >>Visit HPA database.

遺伝子(ID):
ARPC5(10092)
タンパク質の説明:
This gene encodes one of seven subunits of the human Arp2,3 protein complex. The Arp2,3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene,the p16 subunit,has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene.
タンパク質配列:
MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRQGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGSIVRVLTARKTV

研究背景

機能:

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

PTMs:

Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cell projection. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the ARPC5 family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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For Research Use Only.
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