LOX Recombinant Rabbit mAb - #BF3947

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製品: LOX Recombinant Rabbit mAb
カタログ: BF3947
タンパク質の説明: Rabbit monoclonal antibody to LOX
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 54 kDa(Observed); 47kD(Calculated).
ユニプロット: P28300

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製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000-1:5000, IHC 1:200-1:1000, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
クローナリティ:
Monoclonal [ReFirm23394]
特異性:
LOX Recombinant Rabbit mAb detects endogenous levels of LOX.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AAT10

免疫原

免疫原:

A synthetic peptide from human LOX

Uniprot:

P28300(LYOX_HUMAN) >>Visit HPA database.

遺伝子(ID):
LOX(4015)
発現特異性:
P28300 LYOX_HUMAN:

Heart, placenta, skeletal muscle, kidney, lung and pancreas.

タンパク質の説明:
This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants,at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin,while the propeptide may play a role in tumor suppression. In addition,defects in this gene have been linked with predisposition to thoracic aortic aneurysms and dissections.
タンパク質配列:
MRFAWTVLLLGPLQLCALVHCAPPAAGQQQPPREPPAAPGAWRQQIQWENNGQVFSLLSLGSQYQPQRRRDPGAAVPGAANASAQQPRTPILLIRDNRTAAARTRTAGSSGVTAGRPRPTARHWFQAGYSTSRAREAGASRAENQTAPGEVPALSNLRPPSRVDGMVGDDPYNPYKYSDDNPYYNYYDTYERPRPGGRYRPGYGTGYFQYGLPDLVADPYYIQASTYVQKMSMYNLRCAAEENCLASTAYRADVRDYDHRVLLRFPQRVKNQGTSDFLPSRPRYSWEWHSCHQHYHSMDEFSHYDLLDANTQRRVAEGHKASFCLEDTSCDYGYHRRFACTAHTQGLSPGCYDTYGADIDCQWIDITDVKPGNYILKVSVNPSYLVPESDYTNNVVRCDIRYTGHHAYASGCTISPY

研究背景

機能:

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (By similarity).

PTMs:

The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.

Proteolytically cleaved by BMP1 which removes the propeptide. Also proteolytically cleaved by ADAMTS2 and ADAMTS14, but not by ADAMTS3, at an additional cleavage site downstream of the BMP1 cleavage site. The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin (By similarity). Cleavage by BMP1 to remove the propeptide does not increase enzymatic activity but increases binding to collagen. Cleavage by ADAMTS2 produces a form with reduced collagen-binding activity.

Sulfated at Tyr-187 and also at either Tyr-183 or Tyr-184 which enhances binding to collagen.

細胞の位置付け:

Secreted. Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Heart, placenta, skeletal muscle, kidney, lung and pancreas.

タンパク質ファミリー:

Belongs to the lysyl oxidase family.

Restrictive clause

 

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