製品: ACRBP Antibody
カタログ: DF3353
タンパク質の説明: Rabbit polyclonal antibody to ACRBP
アプリケーション: WB IF/ICC
反応性: Human, Rat
予測: Pig, Bovine, Horse, Dog
分子量: 61 KD; 61kD(Calculated).
ユニプロット: Q8NEB7
RRID: AB_2835569

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(89%), Bovine(88%), Horse(88%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
ACRBP Antibody detects endogenous levels of total ACRBP.
RRID:
AB_2835569
引用形式: Affinity Biosciences Cat# DF3353, RRID:AB_2835569.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ACRBP; ACRBP_HUMAN; acrosin binding protein; Acrosin binding protein precursor; Acrosin-binding protein; Cancer/testis antigen 23; Cancer/testis antigen OY TES 1; Cancer/testis antigen OY-TES-1; CT23; FLJ51160; OY TES 1; Proacrosin binding protein sp32; Proacrosin-binding protein sp32; SP32;

免疫原

免疫原:

A synthesized peptide derived from human ACRBP, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q8NEB7 ACRBP_HUMAN:

Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers.

タンパク質配列:
MRKPAAGFLPSLLKVLLLPLAPAAAQDSTQASTPGSPLSPTEYERFFALLTPTWKAETTCRLRATHGCRNPTLVQLDQYENHGLVPDGAVCSNLPYASWFESFCQFTHYRCSNHVYYAKRVLCSQPVSILSPNTLKEIEASAEVSPTTMTSPISPHFTVTERQTFQPWPERLSNNVEELLQSSLSLGGQEQAPEHKQEQGVEHRQEPTQEHKQEEGQKQEEQEEEQEEEGKQEEGQGTKEGREAVSQLQTDSEPKFHSESLSSNPSSFAPRVREVESTPMIMENIQELIRSAQEIDEMNEIYDENSYWRNQNPGSLLQLPHTEALLVLCYSIVENTCIITPTAKAWKYMEEEILGFGKSVCDSLGRRHMSTCALCDFCSLKLEQCHSEASLQRQQCDTSHKTPFVSPLLASQSLSIGNQVGSPESGRFYGLDLYGGLHMDFWCARLATKGCEDVRVSGWLQTEFLSFQDGDFPTKICDTDYIQYPNYCSFKSQQCLMRNRNRKVSRMRCLQNETYSALSPGKSEDVVLRWSQEFSTLTLGQFG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
88
Bovine
88
Dog
88
Rabbit
63
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May be involved in packaging and condensation of the acrosin zymogen in the acrosomal matrix via its association with proacrosin.

PTMs:

The N-terminus is blocked.

Phosphorylated on Tyr residues in capacitated sperm.

細胞の位置付け:

Secreted. Cytoplasmic vesicle>Secretory vesicle>Acrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers.

参考文献

1). Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome. Theriogenology, 2022 (PubMed: 35247785) [IF=2.4]

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