UFM1 Recombinant Rabbit mAb - #BF3925

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製品: UFM1 Recombinant Rabbit mAb
カタログ: BF3925
タンパク質の説明: Rabbit monoclonal antibody to UFM1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 9 kDa(Observed); 9kD(Calculated).
ユニプロット: P61960

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製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
クローナリティ:
Monoclonal [ReFirm23372]
特異性:
UFM1 Recombinant Rabbit mAb detects endogenous levels of UFM1.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BM-002,C13orf20

免疫原

免疫原:

A synthetic peptide from human UFM1

Uniprot:

P61960(UFM1_HUMAN) >>Visit HPA database.

遺伝子(ID):
UFM1(51569)
タンパク質の説明:
UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1,MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M,MIM 603173) (Komatsu et al.,2004 ).
タンパク質配列:
MSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAAEEFKVPAATSAIITNDGIGINPAQTAGNVFLKHGSELRIIPRDRVGSC

研究背景

機能:

Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1. This post-translational modification on lysine residues of proteins may play a crucial role in a number of cellular processes. TRIP4 ufmylation may for instance play a role in nuclear receptors-mediated transcription. Other substrates may include DDRGK1 with which it may play a role in the cellular response to endoplasmic reticulum stress (Probable).

細胞の位置付け:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the UFM1 family.

Restrictive clause

 

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