PRAME Recombinant Rabbit mAb - #BF3917

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製品: PRAME Recombinant Rabbit mAb
カタログ: BF3917
タンパク質の説明: Rabbit monoclonal antibody to PRAME
アプリケーション: WB IHC IF/ICC
反応性: Human
予測: Mouse
分子量: 58 kDa(Observed); 58kD(Calculated).
ユニプロット: P78395

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製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000, IHC 1:100-1:500, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [ReFirm23364]
特異性:
PRAME Recombinant Rabbit mAb detects endogenous levels of PRAME.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

4930534P07Rik; Cancer/testis antigen 130; CT130; MAPE; Melanoma antigen preferentially expressed in tumors; OIP 4; OIP-4; OIP4; OPA interacting protein 4; Opa interacting protein OIP4; OPA-interacting protein 4; PRAME; PRAME_HUMAN; Preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma; RP23-250F8.3;

免疫原

免疫原:

A synthetic peptide from human PRAME

Uniprot:

P78395(PRAME_HUMAN) >>Visit HPA database.

遺伝子(ID):
PRAME(23532)
発現特異性:
P78395 PRAME_HUMAN:

Expressed in testis. Detected in samples of kidney, brain and skin.

タンパク質の説明:
This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues,except testis. The encoded protein acts as a repressor of retinoic acid receptor,and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
タンパク質配列:
MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAMPMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYISPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITDDQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYEDIHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMPN

研究背景

機能:

Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.

細胞の位置付け:

Nucleus. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in testis. Detected in samples of kidney, brain and skin.

タンパク質ファミリー:

Belongs to the PRAME family.

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