ASCL1 Recombinant Rabbit mAb - #BF3893

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製品: ASCL1 Recombinant Rabbit mAb
カタログ: BF3893
タンパク質の説明: Rabbit monoclonal antibody to ASCL1
アプリケーション: IHC
反応性: Human, Mouse
分子量: 25~35 kDa(Observed); 25kD(Calculated).
ユニプロット: P50553

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製品説明

ソース:
Rabbit IgG
アプリケーション:
IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse
クローナリティ:
Monoclonal [ReFirm23340]
特異性:
ASCL1 Recombinant Rabbit mAb detects endogenous levels of ASCL1.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Achaete scute complex homolog 1; Achaete scute complex homolog like 1; Achaete scute complex homologue 1; Achaete scute complex homologue like 1; Achaete scute complex like 1; Achaete scute family bHLH transcription factor 1; Achaete scute protein; Achaete-scute homolog 1; Ascl 1; ascl1; ASCL1_HUMAN; Ash 1; ASH-1; Ash1; bHLHa46; Class A basic helix-loop-helix protein 46; Hash 1; HASH1; Mammalian achaete scute homolog 1; Mammalian achaete scute homologue 1; Mash 1; Mash1;

免疫原

免疫原:

A synthetic peptide from human ASCL1

Uniprot:

P50553(ASCL1_HUMAN) >>Visit HPA database.

遺伝子(ID):
ASCL1(429)
タンパク質の説明:
This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.
タンパク質配列:
MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAADGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTNWF

研究背景

機能:

Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Restrictive clause

 

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