HOXB9 Antibody - #DF3103

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製品: HOXB9 Antibody
カタログ: DF3103
タンパク質の説明: Rabbit polyclonal antibody to HOXB9
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse
予測: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 28 KD(Observed); 28kD(Calculated).
ユニプロット: P17482
RRID: AB_2835480

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#DF3103-BP

HOXB9 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(92%), Bovine(92%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(82%)
クローナリティ:
Polyclonal
特異性:
HOXB9 Antibody detects endogenous levels of total HOXB9.
RRID:
AB_2835480
引用形式: Affinity Biosciences Cat# DF3103, RRID:AB_2835480.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Homeo box 2E; Homeo box B9; Homeobox B9; Homeobox protein Hox B9; Homeobox protein Hox-2.5; Homeobox protein Hox-2E; Homeobox protein Hox-B9; Hox 2.5; Hox 2E; HOX2; HOX2E; Hoxb9; HXB9_HUMAN; OTTHUMP00000216946; OTTHUMP00000243325;

免疫原

免疫原:

A synthesized peptide derived from human HOXB9, corresponding to a region within N-terminal amino acids.

Uniprot:

P17482(HXB9_HUMAN) >>Visit HPA database.

遺伝子(ID):
HOXB9(3219)
タンパク質配列:
MSISGTLSSYYVDSIISHESEDAPPAKFPSGQYASSRQPGHAEHLEFPSCSFQPKAPVFGASWAPLSPHASGSLPSVYHPYIQPQGVPPAESRYLRTWLEPAPRGEAAPGQGQAAVKAEPLLGAPGELLKQGTPEYSLETSAGREAVLSNQRPGYGDNKICEGSEDKERPDQTNPSANWLHARSSRKKRCPYTKYQTLELEKEFLFNMYLTRDRRHEVARLLNLSERQVKIWFQNRRMKMKKMNKEQGKE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Bovine
92
Sheep
92
Dog
92
Rabbit
92
Chicken
82
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the Abd-B homeobox family.

参考文献

1). The roles of HOXB9 and MMP12 in proliferation, migration, and invasion of human laryngeal cancer cells LCC and TU212. Biochemical and biophysical research communications, 2025 (PubMed: 39938450) [IF=2.5]

Application: WB    Species: human    Sample: TU212 cell

Fig. 1. Validation of the transfection effects. (A) Protein levesl of Si-HOXB9. (B) Si-HOXB9 Protenin relative expression. (C) Protein levels of Si-MMP12. (D) SiMMP12 Protenin relative expression. (E) Protein levels of HOXB9-OE. (F) HOXB9-OE protenin relative expression. (G) Protein levels of MMP12-OE. (H) MMP12- OE protenin relative expression. *P < 0.05 vs. NC group.Control. Note: The control group refers to cells treated with nothing, serving as a baseline condition. The NC group refers to cells transfected with a non-targeting siRNA or plasmid vector that does not target any specific gene and is used to control nonspecific effects of the transfection process.

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For Research Use Only.
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