製品: LRAT Antibody
カタログ: DF3055
タンパク質の説明: Rabbit polyclonal antibody to LRAT
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Rabbit, Dog
分子量: 27 KD; 26kD(Calculated).
ユニプロット: O95237
RRID: AB_2835438

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(88%), Horse(88%), Rabbit(100%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
LRAT Antibody detects endogenous levels of total LRAT.
RRID:
AB_2835438
引用形式: Affinity Biosciences Cat# DF3055, RRID:AB_2835438.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

LCA14; Lecithin retinol acyltransferase (phosphatidylcholine retinol O acyltransferase); Lecithin retinol acyltransferase; LRAT; LRAT_HUMAN; Phosphatidylcholine retinol O acyltransferase; Phosphatidylcholine--retinol O-acyltransferase;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O95237 LRAT_HUMAN:

Hepatic stellate cells and endothelial cells (at protein level). Found at high levels in testis and liver, followed by retinal pigment epithelium, small intestine, prostate, pancreas and colon. Low expression observed in brain. In fetal tissues, expressed in retinal pigment epithelium and liver, and barely in the brain.

タンパク質配列:
MKNPMLEVVSLLLEKLLLISNFTLFSSGAAGEDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRSVLASAVLGLASIVCTGLVSYTTLPAIFIPFFLWMAG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
88
Bovine
88
Dog
88
Xenopus
75
Chicken
71
Pig
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95237 基板として

Site PTM Type Enzyme
S10 Phosphorylation
K15 Acetylation
S20 Phosphorylation
Y61 Phosphorylation

研究背景

機能:

Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A (Probable). LRAT plays a critical role in vision (Probable). It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments (Probable). Required for the survival of cone photoreceptors and correct rod photoreceptor cell morphology (By similarity).

細胞の位置付け:

Endoplasmic reticulum membrane>Single-pass membrane protein. Rough endoplasmic reticulum. Endosome>Multivesicular body. Cytoplasm>Perinuclear region.
Note: Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Hepatic stellate cells and endothelial cells (at protein level). Found at high levels in testis and liver, followed by retinal pigment epithelium, small intestine, prostate, pancreas and colon. Low expression observed in brain. In fetal tissues, expressed in retinal pigment epithelium and liver, and barely in the brain.

タンパク質ファミリー:

Belongs to the H-rev107 family.

研究領域

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Organismal Systems > Digestive system > Vitamin digestion and absorption.

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