BCKD Antibody - #AF0314

Fig. 6. Metabolic dysfunction of neuronal PPM1K and BCKDHA increased BCAA levels 24 h after cerebral I/R. a mRNA expression of Slc7a5, Bcat1, Bcat2, Ppm1k, Bckdk and Bckdha at 24 h post cerebral I/R (n = 4–5/group, t test). b The representative picture of immunoblots for BCKD, BCKDK, p-BCKDHA S293, BCKDHA, PPM1K and β-actin at 24 h postcerebral I/R. c Quantification analysis for the immunoblots in (b) (n = 4–6/group, t test). d Representative images of NeuN/PPM1K staining at 24 h post cerebral I/R in sham and I/R groups, including ischemic border (between the two dotted lines) and ischemic core areas. Scale bar = 100 μm. Representative magnified images are on the far right. Scale bar = 50 μm. f Quantification of NeuN-positive and PPM1K-positive cells number (n = 15–18 images from four animals/group, one-way ANOVA followed by multiple comparisons). e Immunofluorescence staining for NeuN/p-BCKDHA S293 at 24 h postcerebral I/R in sham and I/R groups, including ischemic border (between the two dotted lines) and ischemic core areas. Scale bar = 100 μm. Representative magnified images are on the far right. Scale bar = 50 μm. g Quantification of NeuN-positive and p-BCKDHA S293-positive cells (n = 12-18 images from four animals/group, one-way ANOVA followed by multiple comparisons). h BT2 decreased BCAA levels in tissue compared with the VEH group 24 h after reperfusion (n = 8–10/group, one-way ANOVA followed by multiple comparisons). i Quantification of NeuN-positive and p-BCKDHA S293-positive cells (n = 18–21 images from four animals/group, t test). j Immunofluorescence staining of NeuN/p-BCKDHA S293/DAPI in the ischemic core area 24 h postcerebral I/R in VEH and BT2 groups. Scale bar = 50μm. k Representative bands of p-BCKDHA S293 and BCKDHA. l Quantitative analysis of p-BCKDHA S293/BCKDHA protein expression (n = 6/group, t test). All data are presented as mean ± SD, *P