製品: CKI epsilon Antibody
カタログ: DF3188
タンパク質の説明: Rabbit polyclonal antibody to CKI epsilon
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Dog, Chicken, Xenopus
分子量: 47 KD; 47kD(Calculated).
ユニプロット: P49674
RRID: AB_2835411

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Dog(100%), Chicken(83%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
CKI epsilon Antibody detects endogenous levels of total CKI epsilon.
RRID:
AB_2835411
引用形式: Affinity Biosciences Cat# DF3188, RRID:AB_2835411.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Casein kinase 1 epsilon; Casein kinase I; Casein kinase I epsilon; Casein kinase I isoform epsilon; CKI epsilon; CKI-epsilon; CKIe; CKIepsilon; CSNK 1E; CSNK1E; DBT; Doubletime, Drosophila, homolog of; EC 2.7.11.1; epsilon isoform; HCK IE; KC1E; KC1E_HUMAN; kinase CK1 epsilon; MGC 10398;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P49674 KC1E_HUMAN:

Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.

タンパク質配列:
MELRVGNKYRLGRKIGSGSFGDIYLGANIASGEEVAIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSVPFDHLGK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Xenopus
83
Chicken
83
Pig
0
Horse
0
Bovine
0
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49674 基板として

Site PTM Type Enzyme
S17 Phosphorylation
T44 Phosphorylation P49674 (CSNK1E)
K45 Ubiquitination
K54 Ubiquitination
K57 Ubiquitination
K122 Ubiquitination
K130 Ubiquitination
K140 Ubiquitination
K141 Ubiquitination
K171 Ubiquitination
K232 Ubiquitination
K242 Acetylation
K263 Ubiquitination
Y266 Phosphorylation
R321 Methylation
S323 Phosphorylation P49674 (CSNK1E)
T325 Phosphorylation P49674 (CSNK1E)
T334 Phosphorylation P49674 (CSNK1E)
T337 Phosphorylation P49674 (CSNK1E)
R342 Methylation
S343 Phosphorylation
S350 Phosphorylation
T351 Phosphorylation
S354 Phosphorylation
T362 Phosphorylation
S363 Phosphorylation
S368 Phosphorylation P49674 (CSNK1E)
S377 Phosphorylation
R382 Methylation
S389 Phosphorylation Q13131 (PRKAA1)
S390 Phosphorylation
S391 Phosphorylation
T394 Phosphorylation
S400 Phosphorylation
S405 Phosphorylation P49674 (CSNK1E)
T407 Phosphorylation P49674 (CSNK1E)
S408 Phosphorylation P49674 (CSNK1E)

PTMs - P49674 酵素として

Substrate Site Source
O14640 (DVL1) S139 Uniprot
O14640 (DVL1) S142 Uniprot
O14641 (DVL2) S143 Uniprot
O14641 (DVL2) T224 Uniprot
O15055-1 (PER2) S662 Uniprot
O15055-1 (PER2) S665 Uniprot
O15055 (PER2) S668 Uniprot
O15055-1 (PER2) S671 Uniprot
O15055-1 (PER2) S674 Uniprot
O60716 (CTNND1) S268 Uniprot
O75581 (LRP6) S1420 Uniprot
O75581 (LRP6) S1430 Uniprot
O75581 (LRP6) S1431 Uniprot
O95863 (SNAI1) S104 Uniprot
O95863 (SNAI1) S107 Uniprot
P12830 (CDH1) S844 Uniprot
P25054 (APC) S1279 Uniprot
P25054 (APC) S1392 Uniprot
P25054-1 (APC) S1504 Uniprot
P25054-1 (APC) S1505 Uniprot
P25054-1 (APC) S1507 Uniprot
P25054-1 (APC) S1510 Uniprot
P35222 (CTNNB1) S45 Uniprot
P46937 (YAP1) S400 Uniprot
P46937 (YAP1) S403 Uniprot
P49674 (CSNK1E) T44 Uniprot
P49674 (CSNK1E) S323 Uniprot
P49674 (CSNK1E) T325 Uniprot
P49674 (CSNK1E) T334 Uniprot
P49674 (CSNK1E) T337 Uniprot
P49674 (CSNK1E) S368 Uniprot
P49674 (CSNK1E) S405 Uniprot
P49674 (CSNK1E) T407 Uniprot
P49674 (CSNK1E) S408 Uniprot
P55957 (BID) T59 Uniprot
P55957 (BID) S64 Uniprot
P56645-1 (PER3) S622 Uniprot
P56645-1 (PER3) S625 Uniprot
P56645-1 (PER3) S628 Uniprot
P56645-1 (PER3) S633 Uniprot
P56645-1 (PER3) S634 Uniprot
P56645-1 (PER3) T635 Uniprot
Q13541 (EIF4EBP1) T41 Uniprot
Q13541 (EIF4EBP1) T50 Uniprot
Q8TD43 (TRPM4) S839 Uniprot
Q9BZL6 (PRKD2) S244 Uniprot
Q9GZV5 (WWTR1) S314 Uniprot
Q9Y6Q9 (NCOA3) S102 Uniprot

研究背景

機能:

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1 and DVL2. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.

PTMs:

Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.

サブユニット構造:

Monomer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins (By similarity). Interacts with PER1. Interacts with ANKRD6 (By similarity). Interacts with DBNDD2. Interacts with LRP5 and LRP6. Interacts with SOCS3. Interacts with SNAI1 (via zinc fingers). Interacts with DDX3X; the interaction greatly improves CSNK1E affinity for ATP and enhances DVL2 phosphorylation (By similarity).

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

研究領域

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)

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