AFfirm™ DOK4 Mouse Monoclonal Antibody - #BF9311
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
docking protein 4; Downstream of tyrosine kinase 4; FLJ10488; insulin receptor substrate 5; IRS5;
免疫原
Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells.
- Q8TEW6 DOK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATNFSDIVKQGYVKMKSRKLGIYRRCWLVFRKSSSKGPQRLEKYPDEKSVCLRGCPKVTEISNVKCVTRLPKETKRQAVAIIFTDDSARTFTCDSELEAEEWYKTLSVECLGSRLNDISLGEPDLLAPGVQCEQTDRFNVFLLPCPNLDVYGECKLQITHENIYLWDIHNPRVKLVSWPLCSLRRYGRDATRFTFEAGRMCDAGEGLYTFQTQEGEQIYQRVHSATLAIAEQHKRVLLEMEKNVRLLNKGTEHYSYPCTPTTMLPRSAYWHHITGSQNIAEASSYAGEGYGAAQASSETDLLNRFILLKPKPSQGDSSEAKTPSQ
PTMs - Q8TEW6 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y13 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| Y165 | Phosphorylation | Uniprot | |
| Y220 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| Y255 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| Y257 | Phosphorylation | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot |
研究背景
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.
Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation.
Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells.
Interacts with RET and TEK/TIE2. Interaction with RET is mediated through the PTB domain and requires phosphorylation of RET 'Tyr-1062' (By similarity).
PTB domain mediates receptor interaction.
Belongs to the DOK family. Type B subfamily.
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