AFfirm™ MeCP2 Mouse Monoclonal Antibody - #BF8831
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
AUTSX 3; AUTSX3; DKFZp686A24160; Mbd 5; Mbd5; MECP 2; MeCP 2 protein; MeCP-2 protein; Mecp2; MECP2_HUMAN; Methyl CpG binding protein 2 (Rett syndrome); Methyl CpG binding protein 2; Methyl-CpG-binding protein 2; MRX 16; MRX 79; MRX16; MRX79; MRXS 13; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP 10; WBP10;
免疫原
- P51608 MECP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS
PTMs - P51608 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S13 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Q9H2X6 (HIPK2) | Uniprot |
| S86 | Phosphorylation | Uniprot | |
| R115 | Methylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| K119 | Methylation | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| R162 | Methylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K210 | Methylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| K219 | Acetylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| K267 | Methylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K285 | Acetylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| K321 | Acetylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| K347 | Methylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S426 | Phosphorylation | Uniprot | |
| K449 | Acetylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot |
研究背景
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
Nucleus.
Note: Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.
Present in all adult somatic tissues tested.
Interacts with FNBP3 (By similarity). Interacts with CDKL5. Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells (By similarity). Interacts with NCOR2 (By similarity). Interacts with TBL1XR1; bridges interaction between MECP2 and NCOR1. Interacts with TBL1X; recruits TBL1X to the heterochromatin foci.
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