DDX4 Antibody - #AF4098

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製品: DDX4 Antibody
カタログ: AF4098
タンパク質の説明: Rabbit polyclonal antibody to DDX4
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 80kd; 79kD(Calculated).
ユニプロット: Q9NQI0
RRID: AB_2835367

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(92%), Horse(100%), Sheep(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
DDX4 Antibody detects endogenous levels of total DDX4.
RRID:
AB_2835367
引用形式: Affinity Biosciences Cat# AF4098, RRID:AB_2835367.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DDX 4; Ddx4; DDX4_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 4; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MVH; Probable ATP dependent RNA helicase DDX4; Probable ATP-dependent RNA helicase DDX4; VASA; Vasa homolog;

免疫原

免疫原:

A synthesized peptide derived from human DDX4, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9NQI0(DDX4_HUMAN) >>Visit HPA database.

遺伝子(ID):
DDX4(54514)
発現特異性:
Q9NQI0 DDX4_HUMAN:

Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

タンパク質の説明:
May play a role in germ cell development. May play a role in sperm motility.
タンパク質配列:
MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Bovine
92
Rabbit
75
Chicken
50
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).

細胞の位置付け:

Cytoplasm. Cytoplasm>Perinuclear region.
Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

タンパク質ファミリー:

Belongs to the DEAD box helicase family. DDX4/VASA subfamily.

参考文献

1). DDX4 enhances antiviral activity of type I interferon by disrupting interaction of USP7/SOCS1 and promoting degradation of SOCS1. mBio, 2024 (PubMed: 38376239) [IF=5.1]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Fig 1 IFN upregulates the expression of DDX4. (A) Relative mRNA levels of DDX family proteins stimulated by IFN using the Interferome database (http://www.interferome.org/). (B and C) RT-qPCR analysis of Ddx4 mRNA levels in RAW264.7 (B) or MEF (C) cells treated with mIFNβ (300 or 600 IU/mL). (D) RT-qPCR analysis of DDX4 mRNA levels in 2fTGH cells treated with IFNα (1,000 or 3,000 IU/mL). (E) Western blot analysis of DDX4 protein in RAW264.7 cells treated with mIFNβ (300 IU/mL) for the indicated times. (F) Western blot analysis of DDX4 protein in RAW264.7 cells infected with VSV (MOI (Multiplicity Of Infection) = 1.0) for the indicated times. (G and H) Western blot analysis of DDX4 and STAT1 proteins in Stat1+/+ or Stat1-/- RAW264.7 cells treated with mIFNβ (300 IU/mL) (G) or infected with VSV (MOI = 1.0) (H). Data are shown as mean  ±  SD of four biological replicates (B–D) or are representative of three independent experiments (E–H). *P < 0.05 and ***P < 0.001.

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