製品: CNPase Antibody
カタログ: AF4084
タンパク質の説明: Rabbit polyclonal antibody to CNPase
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Monkey
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 47kd; 48kD(Calculated).
ユニプロット: P09543
RRID: AB_2835363

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Monkey
予測:
Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Dog(83%)
クローナリティ:
Polyclonal
特異性:
CNPase Antibody detects endogenous levels of total CNPase.
RRID:
AB_2835363
引用形式: Affinity Biosciences Cat# AF4084, RRID:AB_2835363.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

2''; 2'3' cyclic nucleotide 3' phosphodiesterase; 3''-cyclic-nucleotide 3''-phosphodiesterase; CN37_HUMAN; CNP 1; CNP; CNP1; CNPase;

免疫原

免疫原:

A synthesized peptide derived from human CNPase, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
タンパク質の説明:
Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 2'-phosphate.
タンパク質配列:
MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Horse
83
Bovine
83
Sheep
83
Dog
83
Rabbit
75
Chicken
67
Zebrafish
50
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.

細胞の位置付け:

Membrane>Lipid-anchor. Melanosome.
Note: Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the 2H phosphoesterase superfamily. CNPase family.

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