FOXP4 Antibody - #AF4057

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製品: FOXP4 Antibody
カタログ: AF4057
タンパク質の説明: Rabbit polyclonal antibody to FOXP4
アプリケーション: WB
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 74kd; 73kD(Calculated).
ユニプロット: Q8IVH2
RRID: AB_2835349

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
FOXP4 Antibody detects endogenous levels of total FOXP4.
RRID:
AB_2835349
引用形式: Affinity Biosciences Cat# AF4057, RRID:AB_2835349.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

1200010K03Rik; 2310007G05Rik; FKHLA; FLJ40908; FLJ44184; Fork head related protein like A; Fork head-related protein-like A; Forkhead box P4; Forkhead box protein P4; FOX P4; FOXP 4; foxp4; FOXP4_HUMAN; hFKHLA; mFKHLA; OTTHUMP00000016374; OTTHUMP00000039784; OTTHUMP00000043412; OTTHUMP00000043536; Winged helix repressor FOX P4; Winged helix repressor FOXP 4; Winged helix repressor FOXP4;

免疫原

免疫原:

A synthesized peptide derived from human FOXP4, corresponding to a region within N-terminal amino acids.

Uniprot:

Q8IVH2(FOXP4_HUMAN) >>Visit HPA database.

遺伝子(ID):
FOXP4(116113)
タンパク質の説明:
Transcriptional repressor that represses lung-specific expression.
タンパク質配列:
MMVESASETIRSAPSGQNGVGSLSGQADGSSGGATGTTASGTGREVTTGADSNGEMSPAELLHFQQQQALQVARQFLLQQASGLSSPGNNDSKQSASAVQVPVSVAMMSPQMLTPQQMQQILSPPQLQALLQQQQALMLQQLQEYYKKQQEQLHLQLLTQQQAGKPQPKEALGNKQLAFQQQLLQMQQLQQQHLLNLQRQGLVSLQPNQASGPLQTLPQAAVCPTDLPQLWKGEGAPGQPAEDSVKQEGLDLTGTAATATSFAAPPKVSPPLSHHTLPNGQPTVLTSRRDSSSHEETPGSHPLYGHGECKWPGCETLCEDLGQFIKHLNTEHALDDRSTAQCRVQMQVVQQLEIQLAKESERLQAMMAHLHMRPSEPKPFSQPLNPVPGSSSFSKVTVSAADSFPDGLVHPPTSAAAPVTPLRPPGLGSASLHGGGPARRRSSDKFCSPISSELAQNHEFYKNADVRPPFTYASLIRQAILETPDRQLTLNEIYNWFTRMFAYFRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEREYQKRRPPKMTGSPTLVKNMISGLSYGALNASYQAALAESSFPLLNSPGMLNPGSASSLLPLSHDDVGAPVEPLPSNGSSSPPRLSPPQYSHQVQVKEEPAEAEEDRQPGPPLGAPNPSASGPPEDRDLEEELPGEELS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
58
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Transcriptional repressor that represses lung-specific expression.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

The leucine-zipper is required for dimerization and transcriptional repression.

参考文献

1). Downregulation of microRNA‑423‑5p suppresses TGF‑β1‑induced EMT by targeting FOXP4 in airway fibrosis. Molecular Medicine Reports, 2022 (PubMed: 35642665) [IF=3.4]

Application: IF/ICC    Species:    Sample:

Figure 3. Identification of target genes of miR‑423‑5p. (A‑C) The location of miR‑423‑5p and FOXP4 mRNA in BEAS‑2B cells was determined by fluores‑ cence in situ hybridization (scale bar=200 µm). (D) The binding of miR‑423‑5p and FOXP4 in BEAS‑2B cells was detected by an RNA pull‑down assay. (E) The expression of FOXP4 in AF tissues and TGF‑β1‑stimulated BEAS‑2B cells was detected by immunohistochemistry (scale bar=50 µm) and RT‑qPCR. The relative expression of FOXP4 in BEAS‑2B cells transfected with a miR‑423‑5p mimic or inhibitor for 24 h and then treated with TGF‑β1 for 24 h was determined by (G) RT‑qPCR, (F) immunofluorescence staining (scale bar=50 µm.) and (H) western blot analysis. * P<0.05 and **P<0.01. miR, microRNA; FOXP4, forkhead box p4; RT‑qPCR, reverse transcription‑quantitative PCR; AF, airway fibrosis.

Application: WB    Species: Human    Sample: BEAS‑2B cell

Figure 3. Identification of target genes of miR‑423‑5p. (A‑C) The location of miR‑423‑5p and FOXP4 mRNA in BEAS‑2B cells was determined by fluores‑ cence in situ hybridization (scale bar=200 µm). (D) The binding of miR‑423‑5p and FOXP4 in BEAS‑2B cells was detected by an RNA pull‑down assay. (E) The expression of FOXP4 in AF tissues and TGF‑β1‑stimulated BEAS‑2B cells was detected by immunohistochemistry (scale bar=50 µm) and RT‑qPCR. The relative expression of FOXP4 in BEAS‑2B cells transfected with a miR‑423‑5p mimic or inhibitor for 24 h and then treated with TGF‑β1 for 24 h was determined by (G) RT‑qPCR, (F) immunofluorescence staining (scale bar=50 µm.) and (H) western blot analysis. * P<0.05 and **P<0.01. miR, microRNA; FOXP4, forkhead box p4; RT‑qPCR, reverse transcription‑quantitative PCR; AF, airway fibrosis.

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