N Cadherin Antibody | Affinity Biosciences

製品:	N Cadherin Antibody
カタログ:	AF4039
タンパク質の説明:	Rabbit polyclonal antibody to N Cadherin
アプリケーション:	WB IHC IF/ICC
Cited expt.:	WB, IHC, IF/ICC
反応性:	Human, Mouse, Rat
予測:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	140kd; 100kD(Calculated).
ユニプロット:	P19022
RRID:	AB_2835344

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サイズ	価格	在庫状況
50ul	$250	在庫あり
100ul	$350	在庫あり
200ul	$450	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:1000, IHC 1:50-200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

クローナリティ:

Polyclonal

特異性:

N Cadherin Antibody detects endogenous levels of total N Cadherin.

RRID:

AB_2835344
引用形式: Affinity Biosciences Cat# AF4039, RRID:AB_2835344.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

CADH2_HUMAN; Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; Cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; N-cadherin; NCAD; Neural cadherin; OTTHUMP00000066304; OTTHUMP00000067378;

免疫原

免疫原:

A synthesized peptide derived from human N Cadherin, corresponding to a region within C-terminal amino acids.

Uniprot:

P19022(CADH2_HUMAN) >>Visit HPA database.

遺伝子(ID):

CDH2(1000)

タンパク質の説明:

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density

タンパク質配列:

P19022 CADH2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MCRIAGALRTLLPLLAALLQASVEASGEIALCKTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNAVYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIVGAGLGTGAIIAILLCIIILLILVLMFVVWMKRRDKERQAKQLLIDPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPDTVEPDAIKPVGIRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGLKAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKKLADMYGGGDD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

P19022_CADH2_HUMAN

機能:

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

PTMs:

Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).

May be phosphorylated by OBSCN.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein. Cell membrane>Sarcolemma. Cell junction. Cell surface.
Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes.

タンパク質ファミリー:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Calcium-binding sites are occupied sequentially in the order of site 3, then site 2 and site 1.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

参考文献

1). Methyltransferase like 13 mediates the translation of Snail in head and neck squamous cell carcinoma. International journal of oral science, 2021 (PubMed: 34381012) [IF=10.8]

2). METTL13 Mediates the Translation of Snail in Head and Neck Squamous Cell Carcinoma. International Journal of Oral Science, 2020 (PubMed: 34381012) [IF=10.8]

3). Roles of gut microbiome-associated metabolites in pulmonary fibrosis by integrated analysis. NPJ biofilms and microbiomes, 2024 (PubMed: 39702426) [IF=9.2]

4). METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner. Cell death & disease, 2022 (PubMed: 35985997) [IF=8.1]

Application: WB Species: human Sample: BPH-1 cells

Fig. 3: Effects of METTL3 knockdown on proliferation, apoptosis, and EMT of TGF-β-treated BPH-1 cells. METTL3 knockdown was achieved in TGF-β-treated BPH-1 cells by transducing Lv-sh-METTL3 and then examined for A, B METTL3 expression using qRT-PCR (A) and Immunoblotting (B); C m6A status in RNA using an m6A RNA Methylation Assay Kit; D cell viability using MTT assay; E DNA synthesis using EdU assay; Scale bar = 20 μm; F the protein levels of E-cadherin, N-cadherin, vimentin using Immunoblotting; G cell apoptosis using flow cytometry; H the protein levels of Bcl-2, Bax, Caspase 9, cleaved caspase 9, Caspase 3, cleaved caspase 3, and cleaved PARP-1 using Immunoblotting. **P

5). Huaier polysaccharides suppress triple-negative breast cancer metastasis and epithelial-mesenchymal transition by inducing autophagic degradation of Snail. Cell and Bioscience, 2021 (PubMed: 34481526) [IF=7.5]

Application: WB Species: human Sample: breast cancer cells

Fig. 1| PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantifed using the NIH ImageJ software. (mean ± SD, *P < 0.05 and **P < 0.01)

Application: WB Species: Human Sample: breast cancer cells

Fig. 1 PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. a The invasiveness of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at 5 μg/mL for 12 h is evaluated using Transwell invasion assays at 24 h (mean ± SD, ***P < 0.001). b Migratory ability of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at different concentrations is evaluated using scratch assays at different time points (mean ± SD, **P < 0.01, ***P < 0.001). c Mice are treated with normal saline (control), 25 μg/g or 100 μg/g PS-T by oral gavage every other day for 21 days (mean ± SD, *P < 0.05, **P < 0.01, scale bars: 100 μm). d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantified using the NIH ImageJ software. (mean ± SD, *P < 0.05 and **P < 0.01)

6). TRIP13 interference inhibits the proliferation and metastasis of thyroid cancer cells through regulating TTC5/p53 pathway and epithelial-mesenchymal transition related genes expression. BIOMEDICINE & PHARMACOTHERAPY, 2019 (PubMed: 31648166) [IF=6.9]

7). d-Borneol enhances cisplatin sensitivity via autophagy dependent EMT signaling and NCOA4-mediated ferritinophagy. PHYTOMEDICINE, 2022 (PubMed: 36030746) [IF=6.7]

8). Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. LIVER INTERNATIONAL, 2022 (PubMed: 34854209) [IF=6.0]

Application: WB Species: Human Sample: HepG2 and Huh7 cells

FIGURE 7 A, Representative images of the western blot results for uPAR, MMP2 and MMP9 in ALB knockdown HepG2 and Huh7 cells; B, Zymography analysis illustrates MMP2 and MMP9 activity in ALB knockdown HepG2 and Huh7 cells; C, Quantitative analysis results and representative images of the western blot results for the EMT‐associated markers, E‐cadherin, N‐cadherin, vimentin, Snail and Twist by western blot in ALB knockdown HepG2 and Huh7 cells; D, Quantification shows a significantly higher uPAR in HCC group with ALB <3.5 g/dL compared to ALB ≥3.5 g/dL (*P < .05); E, Scatterplot showing the correlation between plasma levels of ALB and uPAR. The vertical position represents the expression levels of uPAR (lg pg/mL)

9). Elevated expression of HIGD1A drives hepatocellular carcinoma progression by regulating polyamine metabolism through c-Myc-ODC1 nexus. Cancer & metabolism, 2024 (PubMed: 38395945) [IF=5.9]

Application: WB Species: human Sample: L02, HepG2, Huh7, and MHCC97H cells

Fig. 3 HIGD1A knockdown inhibits the migration and invasion of HCC cells. A Cell migration ability of L02, HepG2, Huh7, and MHCC97H cells infected with shCtrl or shHIGD1A lentivirus was analyzed by transwell assay. Representative images of the transwell assay (left) and quantitative data of migrated cells (right) are shown. B Cell invasion ability of L02, HepG2, Huh7, and MHCC97H cells infected with shCtrl or shHIGD1A lentivirus as analyzed by transwell assay. Representative images of the transwell assay (left) and quantitative data of migrated cells (right) are shown. C Expression of EMT markers E-cadherin and N-cadherin in L02, HepG2, Huh7, and MHCC97H cells infected with shCtrl or shHIGD1A lentivirus as examined by western blotting. Results shown are mean ± SEM. *P

10). Polydopamine/carboxylic graphene oxide-composited polypyrrole films for promoting adhesion and alignment of Schwann cells. Colloids and surfaces-B, Biointerfaces, 2020 (PubMed: 32203860) [IF=5.4]

Application: WB Species: Sample: RSCs

Fig. 3.| Cell images of immunofluorescence staining for (a) N-cadherin expression, phalloidin, (b) GFAP, (c) S100 and DAPI, and scale bar is 50 μm. (d) WB assay for Vinculin, Ncadherin, P75 and GFAP proteins and (e) their semi-quantitative comparison. & shows p＜0.05, compared with control; # shows p＜0.05, compared with PPy-PLLA; Δ shows p＜0.05, compared with CGO/PPy-PLLA.

Application: IF/ICC Species: Sample: RSCs

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N Cadherin Antibody - #AF4039

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