製品: MAP3K8/COT Mouse Monoclonal Antibody
カタログ: BF8123
タンパク質の説明: Mouse monoclonal antibody to MAP3K8/COT
アプリケーション: WB
反応性: Human, Mouse
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 60kDa; 53kD(Calculated).
ユニプロット: P41279

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Monoclonal [AFfirm8123]
特異性:
MAP3K8/COT Antibody detects endogenous levels of total MAP3K8/COT.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

AURA2; c COT; Cancer Osaka thyroid oncogene; CCOT; COT; COT proto oncogene serine/threonine protein kinase; EST; ESTF; Ewing sarcoma transformant; FLJ10486; M3K8_HUMAN; MAP3K 8; MAP3K8; MEKK8; Mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Proto oncogene cCot; Proto-oncogene c-Cot; Serine/threonine protein kinase cot; Serine/threonine-protein kinase cot; TPL 2; TPL-2; TPL2; Tumor progression locus 2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P41279 M3K8_HUMAN:

Expressed in several normal tissues and human tumor-derived cell lines.

タンパク質の説明:
This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways.
タンパク質配列:
MEYMSTGSDNKEEIDLLIKHLNVSDVIDIMENLYASEEPAVYEPSLMTMCQDSNQNDERSKSLLLSGQEVPWLSSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKLIPVDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEALNPPREDQPRCQSLDSALLERKRLLSRKELELPENIADSSCTGSTEESEMLKRQRSLYIDLGALAGYFNLVRGPPTLEYG

PTMs - P41279 基板として

Site PTM Type Enzyme
S62 Phosphorylation
T80 Phosphorylation
S125 Phosphorylation
Y136 Phosphorylation
S141 Phosphorylation
Y153 Phosphorylation
T290 Phosphorylation
S334 Phosphorylation
S368 Phosphorylation
S400 Phosphorylation P31749 (AKT1) , Q9P1W9 (PIM2)
S413 Phosphorylation Q9P1W9 (PIM2) , P31749 (AKT1)
S443 Phosphorylation P41279 (MAP3K8)

PTMs - P41279 酵素として

Substrate Site Source
O14757 (CHEK1) S345 Uniprot
P00519 (ABL1) T735 Uniprot
P06493 (CDK1) T161 Uniprot
P06748 (NPM1) T199 Uniprot
P15311 (EZR) T567 Uniprot
P16220 (CREB1) S133 Uniprot
P26038 (MSN) T558 Uniprot
P35241 (RDX) T564 Uniprot
P36507 (MAP2K2) S222 Uniprot
P36507 (MAP2K2) S226 Uniprot
P41279 (MAP3K8) S443 Uniprot
P46734 (MAP2K3) S218 Uniprot
P52564 (MAP2K6) S207 Uniprot
P53350 (PLK1) S137 Uniprot
P53350 (PLK1) T210 Uniprot
Q02750 (MAP2K1) S218 Uniprot
Q02750 (MAP2K1) S222 Uniprot
Q02750 (MAP2K1) S244 Uniprot
Q02750 (MAP2K1) S248 Uniprot
Q04637 (EIF4G1) S1147 Uniprot
Q13526 (PIN1) S16 Uniprot
Q13541 (EIF4EBP1) T70 Uniprot
Q15418 (RPS6KA1) S380 Uniprot
Q99558 (MAP3K14) T559 Uniprot

研究背景

機能:

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

PTMs:

Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Phosphorylated on Thr-290; the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Phosphorylated on Ser-400 by IKBKB; the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in several normal tissues and human tumor-derived cell lines.

サブユニット構造:

Forms a ternary complex with NFKB1/p105 and TNIP2. Interacts with NFKB1; the interaction increases the stability of MAP3K8 but inhibits its MEK phosphorylation activity, whereas loss of interaction following LPS stimulation leads to its degradation. Interacts with CD40 and TRAF6; the interaction is required for ERK activation. Interacts with KSR2; the interaction inhibits ERK and NF-kappa-B activation.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

研究領域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

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