製品: Glutamine Synthetase Mouse Monoclonal Antibody
カタログ: BF8094
タンパク質の説明: Mouse monoclonal antibody to Glutamine Synthetase
アプリケーション: IHC
反応性: Human
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 42 kDa; 42kD(Calculated).
ユニプロット: P15104

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Mouse
アプリケーション:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFfirm8094]
特異性:
Glutamine Synthetase Antibody detects endogenous levels of total Glutamine Synthetase.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

cell proliferation-inducing protein 59; GLNA; GLNA_HUMAN; GLNS; GLUL; Glutamate ammonia ligase; Glutamate decarboxylase; Glutamate--ammonia ligase; glutamine synthase; Glutamine synthetase; GS; PIG 43; PIG 59; PIG43; PIG59; Proliferation inducing protein 43;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P15104 GLNA_HUMAN:

Expressed in endothelial cells.

タンパク質配列:
MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN

PTMs - P15104 基板として

Site PTM Type Enzyme
T2 Acetylation
K11 Acetylation
K11 Ubiquitination
K14 Acetylation
K14 Ubiquitination
Y17 Phosphorylation
S19 Phosphorylation
K25 Ubiquitination
K52 Ubiquitination
K91 Ubiquitination
K95 Ubiquitination
K103 Acetylation
K103 Ubiquitination
Y104 Phosphorylation
Y180 Phosphorylation
C183 S-Nitrosylation
Y185 Phosphorylation
K259 Ubiquitination
K268 Ubiquitination
Y269 Phosphorylation
K276 Ubiquitination
K291 Ubiquitination
T301 Phosphorylation
S320 Phosphorylation
S322 Phosphorylation
K334 Ubiquitination
Y336 Phosphorylation
S343 Phosphorylation
C359 S-Nitrosylation
Y371 Phosphorylation
K372 Acetylation
K372 Ubiquitination

研究背景

機能:

Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis.

PTMs:

Palmitoylated; undergoes autopalmitoylation.

Ubiquitinated by ZNRF1.

細胞の位置付け:

Cytoplasm>Cytosol. Microsome. Mitochondrion. Cell membrane>Lipid-anchor.
Note: Mainly localizes in the cytosol, with a fraction associated with the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in endothelial cells.

サブユニット構造:

Decamer; composed of two pentamers. Interacts with PALMD (By similarity). Interacts with RHOJ.

タンパク質ファミリー:

Belongs to the glutamine synthetase family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Energy metabolism > Nitrogen metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

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