製品: TLS/FUS Mouse Monoclonal Antibody
カタログ: BF8048
タンパク質の説明: Mouse monoclonal antibody to TLS/FUS
アプリケーション: WB IHC
反応性: Human, Mouse
予測: Dog, Chicken, Xenopus
分子量: 53 kDa; 53kD(Calculated).
ユニプロット: P35637

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Monoclonal [AFfirm8048(AFP21845)]
特異性:
TLS/FUS Antibody detects endogenous levels of total TLS/FUS.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

75 kDa DNA pairing protein; 75 kDa DNA-pairing protein; ALS6; Amyotrophic lateral sclerosis 6; fus; FUS CHOP; Fus like protein; FUS_HUMAN; FUS1; Fused in sarcoma; Fusion (involved in t(12;16) in malignant liposarcoma); Fusion derived from t(12;16) malignant liposarcoma; Fusion gene in myxoid liposarcoma; Heterogeneous nuclear ribonucleoprotein P2; hnRNP P2; hnRNPP2; Oncogene FUS; Oncogene TLS; POMp75; RNA binding protein FUS; RNA-binding protein FUS; TLS; TLS CHOP; Translocated in liposarcoma; Translocated in liposarcoma protein;

免疫原

免疫原:

A synthesized peptide derived from human TLS/FUS, corresponding to a region within C-terminal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
P35637 FUS_HUMAN:

Ubiquitous.

タンパク質配列:
MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGGRGRGGSGGGGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNSDNNTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY

PTMs - P35637 基板として

Site PTM Type Enzyme
T7 Phosphorylation
T11 Phosphorylation
T19 O-Glycosylation
T19 Phosphorylation
S26 Phosphorylation P78527 (PRKDC)
S30 Phosphorylation
S42 Phosphorylation P78527 (PRKDC) , Q13315 (ATM)
S54 Phosphorylation
S61 Phosphorylation P78527 (PRKDC)
T68 Phosphorylation
S84 Phosphorylation P78527 (PRKDC)
S87 Phosphorylation
S112 Phosphorylation
S117 Phosphorylation
S131 Phosphorylation P78527 (PRKDC)
S142 Phosphorylation
R213 Methylation
R216 Methylation
R218 Methylation
S221 Phosphorylation
Y232 Phosphorylation
R234 Methylation
R242 Methylation
R244 Methylation
R248 Methylation
R251 Methylation
S257 Phosphorylation P05771-2 (PRKCB)
R259 Methylation
K264 Ubiquitination
R269 Methylation
S273 Phosphorylation
S277 Phosphorylation
T286 Phosphorylation
K312 Ubiquitination
K316 Ubiquitination
T317 Phosphorylation
Y325 Phosphorylation
T326 Phosphorylation
K332 Acetylation
K334 Ubiquitination
S340 Phosphorylation
S346 Phosphorylation
K348 Sumoylation
K348 Ubiquitination
K357 Acetylation
K357 Sumoylation
K357 Ubiquitination
S360 Phosphorylation
K365 Methylation
K365 Sumoylation
K365 Ubiquitination
R371 Methylation
R372 Methylation
R377 Methylation
R383 Methylation
R386 Methylation
R388 Methylation
R394 Methylation
Y397 Phosphorylation
R407 Methylation
S439 Phosphorylation
K448 Ubiquitination
S462 Phosphorylation
Y468 Phosphorylation
R473 Methylation
R476 Methylation
R481 Methylation
R485 Methylation
R487 Methylation
R491 Methylation
R495 Methylation
R498 Methylation
R503 Methylation
R514 Methylation
Y526 Phosphorylation P12931 (SRC) , P06241 (FYN)

研究背景

機能:

DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis (By similarity).

PTMs:

Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.

Phosphorylated in its N-terminal serine residues upon induced DNA damage. ATM and DNA-PK are able to phosphorylate FUS N-terminal region.

細胞の位置付け:

Nucleus.
Note: Displays a punctate pattern inside the nucleus and is excluded from nucleoli.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

サブユニット構造:

Self-oligomerizes (via N-terminal region). Oligomerization is essential for chromatin binding. Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP. Interacts with LRSAM1. Interacts with SAFB1 in a DNA-dependent manner; this interaction tethers FUS to chromatin. Interacts with MATR3. Interacts with SNRNP70 and POLR2A; these interactions couple RNA transcription and splicing. Interacts (through its RNA-binding domain) with RALY (through its RNA-binding domain); both are components of the same RNPs.

タンパク質ファミリー:

Belongs to the RRM TET family.

研究領域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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