製品: Ubiquitin Mouse Monoclonal Antibody
カタログ: BF8034
タンパク質の説明: Mouse monoclonal antibody to Ubiquitin
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 8kD,16kD,24kD,32kD; 15kD(Calculated).
ユニプロット: P62987

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Monoclonal [AFfirm8034(AFP21827)]
特異性:
Ubiquitin Antibody detects endogenous levels of total Ubiquitin.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

60S ribosomal protein L40; CEP52; HUBCEP52; L40; MGC126879; MGC57125; RPL40; UBA 52; Ubiquitin 52 amino acid fusion protein; Ubiquitin 60S ribosomal protein L40; Ubiquitin A 52 residue ribosomal protein fusion product 1; Ubiquitin carboxyl extension protein 52; Ubiquitin CEP52; UBA52;

免疫原

免疫原:

A synthesized peptide derived from human Ubiquitin.

Uniprot:
遺伝子(ID):
タンパク質配列:
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLRQLAQKYNCDKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKVK

PTMs - P62987 基板として

Site PTM Type Enzyme
K6 Acetylation
K6 Methylation
K6 Sumoylation
K6 Ubiquitination
T7 Phosphorylation
K11 Acetylation
K11 Sumoylation
K11 Ubiquitination
T12 Phosphorylation
T14 Phosphorylation
S20 Phosphorylation
T22 Phosphorylation
K27 Sumoylation
K27 Ubiquitination
K29 Sumoylation
K29 Ubiquitination
K33 Sumoylation
K33 Ubiquitination
K48 Acetylation
K48 Sumoylation
K48 Ubiquitination
S57 Phosphorylation
Y59 Phosphorylation
K63 Methylation
K63 Sumoylation
K63 Ubiquitination
S65 Phosphorylation
T66 Phosphorylation
R72 Methylation
S81 Phosphorylation
K88 Acetylation
K88 Ubiquitination
C91 S-Nitrosylation
K93 Acetylation
K93 Ubiquitination
K98 Methylation
C115 S-Nitrosylation

研究背景

機能:

Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Component of the 60S subunit of the ribosome. Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs.

PTMs:

Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30.

Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.

細胞の位置付け:

Cytoplasm. Nucleus.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Ribosomal protein L40 is part of the 60S ribosomal subunit. Interacts with UBQLN1 (via UBA domain).

タンパク質ファミリー:

In the N-terminal section; belongs to the ubiquitin family.

In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.

研究領域

· Genetic Information Processing > Translation > Ribosome.

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