製品: Syntaxin 1A Antibody
カタログ: AF6463
タンパク質の説明: Rabbit polyclonal antibody to Syntaxin 1A
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Sheep
分子量: 35kDa; 33kD(Calculated).
ユニプロット: Q16623
RRID: AB_2835283

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Sheep(100%)
クローナリティ:
Polyclonal
特異性:
Syntaxin 1A Antibody detects endogenous levels of total Syntaxin 1A.
RRID:
AB_2835283
引用形式: Affinity Biosciences Cat# AF6463, RRID:AB_2835283.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

HPC 1; Neuron specific antigen HPC1; Neuron-specific antigen HPC-1; OTTHUMP00000174615; OTTHUMP00000174616; OTTHUMP00000174617; OTTHUMP00000174618; P35-1; STX1; STX1A; STX1A_HUMAN; SYN1A; Syntaxin 1A (brain); Syntaxin 1A brain; Syntaxin-1A;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q16623 STX1A_HUMAN:

Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.

タンパク質の説明:
STX1A is a type IV membrane protein involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. Three splice variant isoforms have been described.
タンパク質配列:
MKDRTQELRTAKDSDDDDDVAVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQGEMIDRIEYNVEHAVDYVERAVSDTKKAVKYQSKARRKKIMIIICCVILGIVIASTVGGIFA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16623 基板として

Site PTM Type Enzyme
S14 Phosphorylation P68400 (CSNK2A1)
T23 Phosphorylation
S64 Phosphorylation
S99 Phosphorylation
C145 S-Nitrosylation
S188 Phosphorylation P53355 (DAPK1)
S249 Phosphorylation
S281 Phosphorylation
T282 Phosphorylation

研究背景

機能:

Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Plays also an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (By similarity).

PTMs:

Phosphorylated by CK2 (By similarity). Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1.

Sumoylated, sumoylation is required for regulation of synaptic vesicle endocytosis.

細胞の位置付け:

Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Single-pass type IV membrane protein. Cell junction>Synapse>Synaptosome. Cell membrane.
Note: Colocalizes with KCNB1 at the cell membrane.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.

サブユニット構造:

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex constitutes the basic catalytic machinery of the complex neurotransmitter release apparatus. The SNARE complex interacts with CPLX1 (By similarity). Interacts with STXBP1. Interacts (via C-terminus) with KCNB1 (via C-terminus); the interaction increases in a calcium-dependent manner and induces a pore-independent enhancement of exocytosis in neuroendocrine cells, chromaffin cells, pancreatic beta cells and from the soma of dorsal root ganglia (DRG) neurons (By similarity). Interacts with SYTL4 (By similarity). Interacts with STXBP6 (By similarity). Interacts with PLCL1 (via C2 domain) (By similarity). Interacts with OTOF (By similarity). Interacts with LGI3 (By similarity). Interacts with SLC6A4 (By similarity). Interacts with SYT6 and SYT8; the interaction is Ca(2+)-dependent (By similarity). Interacts with VAMP8. Interacts with SNAP23. Interacts with VAPA and SYBU. Interacts with PRRT2 (By similarity). Interacts with SEPT8 (By similarity). Interacts with STXBP5L (By similarity). Interacts with synaptotagmin-1/SYT1 (By similarity).

タンパク質ファミリー:

Belongs to the syntaxin family.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

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