ERCC6L Antibody - #DF15440

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製品: ERCC6L Antibody
カタログ: DF15440
タンパク質の説明: Rabbit polyclonal antibody to ERCC6L
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human
分子量: 141kD(Calculated).
ユニプロット: Q2NKX8

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MS Report
HPLC Report
MSDS
説明書
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
ERCC6L Antibody detects endogenous levels of ERCC6L.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ATP dependent helicase ERCC6 like; ATP-dependent helicase ERCC6-like; DNA excision repair protein ERCC 6 like; DNA excision repair protein ERCC-6-like; ERC6L_HUMAN; ERCC 6L; Ercc6l; Excision repair cross complementing rodent repair deficiency complementation group 6 like; Excision repair protein ERCC6 like; FLJ20105; MGC131695; PICH; Plk1 interacting checkpoint helicase; PLK1-interacting checkpoint helicase; SNF2/RAD54 family protein; Tumor antigen BJ HCC 15; Tumor antigen BJ-HCC-15;

免疫原

免疫原:

A synthesized peptide derived from human ERCC6L.

Uniprot:

Q2NKX8(ERC6L_HUMAN) >>Visit HPA database.

遺伝子(ID):
ERCC6L(54821)
タンパク質配列:
MEASRRFPEAEALSPEQAAHYLRYVKEAKEATKNGDLEEAFKLFNLAKDIFPNEKVLSRIQKIQEALEELAEQGDDEFTDVCNSGLLLYRELHNQLFEHQKEGIAFLYSLYRDGRKGGILADDMGLGKTVQIIAFLSGMFDASLVNHVLLIMPTNLINTWVKEFIKWTPGMRVKTFHGPSKDERTRNLNRIQQRNGVIITTYQMLINNWQQLSSFRGQEFVWDYVILDEAHKIKTSSTKSAICARAIPASNRLLLTGTPIQNNLQELWSLFDFACQGSLLGTLKTFKMEYENPITRAREKDATPGEKALGFKISENLMAIIKPYFLRRTKEDVQKKKSSNPEARLNEKNPDVDAICEMPSLSRKNDLIIWIRLVPLQEEIYRKFVSLDHIKELLMETRSPLAELGVLKKLCDHPRLLSARACCLLNLGTFSAQDGNEGEDSPDVDHIDQVTDDTLMEESGKMIFLMDLLKRLRDEGHQTLVFSQSRQILNIIERLLKNRHFKTLRIDGTVTHLLEREKRINLFQQNKDYSVFLLTTQVGGVGLTLTAATRVVIFDPSWNPATDAQAVDRVYRIGQKENVVVYRLITCGTVEEKIYRRQVFKDSLIRQTTGEKKNPFRYFSKQELRELFTIEDLQNSVTQLQLQSLHAAQRKSDIKLDEHIAYLQSLGIAGISDHDLMYTCDLSVKEELDVVEESHYIQQRVQKAQFLVEFESQNKEFLMEQQRTRNEGAWLREPVFPSSTKKKCPKLNKPQPQPSPLLSTHHTQEEDISSKMASVVIDDLPKEGEKQDLSSIKVNVTTLQDGKGTGSADSIATLPKGFGSVEELCTNSSLGMEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN

研究背景

機能:

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. Functions as ATP-dependent DNA translocase. Can promote Holliday junction branch migration (in vitro).

PTMs:

Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.

細胞の位置付け:

Chromosome>Centromere. Chromosome>Centromere>Kinetochore. Chromosome.
Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the SNF2/RAD54 helicase family.

参考文献

1). PICH activates Cyclin A1 transcription to drive S-phase progression and chemoresistance in gastric cancer.. Cancer Research, 2023 (PubMed: 37646571) [IF=12.5]
2). Targeted Inhibition of cGAS/STING signaling induced by aberrant R-Loops in the nucleus pulposus to alleviate cellular senescence and intervertebral disc degeneration. Journal of nanobiotechnology, 2025 (PubMed: 40660286) [IF=10.2]

Application: IF/ICC    Species: human    Sample:

Fig. 4 Bulk RNA sequencing identified the critical regulators of R-Loop during IVDD A: Illustration of the critical regulator for R-Loops based on bulk RNA sequencing (GSE245147). B: Overlapping R-Loop-related genes between human IVD tissue and NPCs. C: Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed overlapping R-Loop-related genes. D: Protein-protein interaction network of differentially expressed overlapping R-Loop-related genes constructed using the STRING database (https://cn.string-db.org). E: Bar graphs showing fold changes of the expression of ERCC5 and ERCC6 in human IVD tissue and NPCs, respectively (GSE245147). F: Histological analysis (SOFG staining and immunohistochemistry (IHC) staining for ERCC5 and ERCC6) of human NP tissue with varying degeneration grades. Scale bar = 20 μm. G: Quantitative results of IHC staining for ERCC5 and ERCC6 (n = 5). H: Representative images of IF staining for ERCC5 in H2O2-treated primary rat NPCs. Scale bar = 20 μm. I: Quantitative results of IF staining for ERCC5 (n = 3). J: Representative images of IF staining for ERCC6 in H2O2-treated primary rat NPCs. Scale bar = 20 μm. K: Quantitative results of IF staining for ERCC6 (n = 3). Significance was calculated by (G, I, and K) Student’s t-test. *p 

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