OSBPL11 Antibody - #DF15433

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製品: OSBPL11 Antibody
カタログ: DF15433
タンパク質の説明: Rabbit polyclonal antibody to OSBPL11
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse
分子量: 84kD(Calculated).
ユニプロット: Q9BXB4

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
OSBPL11 Antibody detects endogenous levels of OSBPL11.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ13012; FLJ13164; ORP 11; ORP-11; ORP11; OSB11_HUMAN; OSBP related protein 11; OSBP-related protein 11; OSBP12; OSBPL11; OTTHUMP00000215865; Oxysterol binding protein like 11; Oxysterol binding protein related protein 11; Oxysterol-binding protein-related protein 11; TCCCIA00292;

免疫原

免疫原:

A synthesized peptide derived from human OSBPL11.

Uniprot:

Q9BXB4(OSB11_HUMAN) >>Visit HPA database.

遺伝子(ID):
OSBPL11(114885)
発現特異性:
Q9BXB4 OSB11_HUMAN:

Present at highest levels in ovary, testis, kidney, liver, stomach, brain, and adipose tissue. Strong expression (at protein level) in epithelial cells of kidney tubules, testicular tubules, caecum, and skin (PubMed:20599956). Present at low levels in subcutaneous and visceral adipose tissue (at protein level)(PubMed:23028956).

タンパク質配列:
MQGGEPVSTMKVSESEGKLEGQATAVTPNKNSSCGGGISSSSSSRGGSAKGWQYSDHMENVYGYLMKYTNLVTGWQYRFFVLNNEAGLLEYFVNEQSRNQKPRGTLQLAGAVISPSDEDSHTFTVNAASGEQYKLRATDAKERQHWVSRLQICTQHHTEAIGKNNPPLKSRSFSLASSSNSPISQRRPSQNAISFFNVGHSKLQSLSKRTNLPPDHLVEVREMMSHAEGQQRDLIRRIECLPTSGHLSSLDQDLLMLKATSMATMNCLNDCFHILQLQHASHQKGSLPSGTTIEWLEPKISLSNHYKNGADQPFATDQSKPVAVPEEQPVAESGLLAREPEEINADDEIEDTCDHKEDDLGAVEEQRSVILHLLSQLKLGMDLTRVVLPTFILEKRSLLEMYADFMSHPDLFIAITNGATAEDRMIRFVEYYLTSFHEGRKGAIAKKPYNPIIGETFHCSWKMPKSEVASSVFSSSSTQGVTNHAPLSGESLTQVGSDCYTVRFVAEQVSHHPPVSGFYAECTERKMCVNAHVWTKSKFLGMSIGVTMVGEGILSLLEHGEEYTFSLPCAYARSILTVPWVELGGKVSVNCAKTGYSASITFHTKPFYGGKLHRVTAEVKHNITNTVVCRVQGEWNSVLEFTYSNGETKYVDLTKLAVTKKRVRPLEKQDPFESRRLWKNVTDSLRESEIDKATEHKHTLEERQRTEERHRTETGTPWKTKYFIKEGDGWVYHKPLWKIIPTTQPAE

研究背景

機能:

Plays a role in regulating ADIPOQ and FABP4 levels in differentiating adipocytes and is also involved in regulation of adipocyte triglyceride storage. Weakly binds 25-hydroxycholesterol.

細胞の位置付け:

Late endosome membrane. Golgi apparatus>trans-Golgi network membrane.
Note: Localizes at the Golgi-late endosome interface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Present at highest levels in ovary, testis, kidney, liver, stomach, brain, and adipose tissue. Strong expression (at protein level) in epithelial cells of kidney tubules, testicular tubules, caecum, and skin. Present at low levels in subcutaneous and visceral adipose tissue (at protein level).

タンパク質ファミリー:

The PH domain binds phosphoinositides.

Belongs to the OSBP family.

参考文献

1). High level expression of OSBPL11 is associated with atherosclerosis and Alzheimer's disease. Inflammation, 2025 (PubMed: 40407857) [IF=4.5]

Application: IF/ICC    Species: human    Sample:

Fig. 3 Increased expression of OSBPL11 in AS and AD patients. (A) HE and Masson staining of arterial vessels in healthy control and AS patients (Scale bar: 200 μm). (B) Immunohistochemical staining for OSBPL11 expression in healthy control and AS patients (brown-yellow indicates positive staining area, from left to right magnification: 4x; 40x, **P 

Application: WB    Species: Mouse    Sample:

Fig. 5 Increased expression of OSBPL11 in high fat diet fed ApoE−/− mice. (A) Co-localization of OSBPL11 with macrophages in the aorta of ApoE−/− mice (Right fluorescence plot is a merge of the left single-channel fluorescence plot; Scale bar: 75 μm, DAPI in blue fluorescence indicates cell nuclei, CD68 in red fluorescence indicates macrophages, OSBPL11 in green, n = 6). (B) Co-localization of OSBPL11 with astrocytes in the brain of ApoE−/− mice (Scale bar: 150 μm; DAPI in blue fluorescence indicates cell nuclei, GFAP in red fluorescence indicates astrocytes, OSBPL11 in green, n = 6). (C-D) Immunohistochemical staining for OSBPL11 expression in the aorta and the brain of ApoE−/− mice (left to right magnification: 4x; 40x, n = 6). (E–F) Real-time quantitative PCR was performed to detect the expression of OSBPL11 in the aorta and the brain of ApoE−/− mice. (G-H) Western blotting shows the expression of OSBPL11 in the aorta and the brain of ApoE−/− mice. (**P 

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