HSL Antibody - #AF6403

(15)   (8)  
製品: HSL Antibody
カタログ: AF6403
タンパク質の説明: Rabbit polyclonal antibody to HSL
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 85kDa; 117kD(Calculated).
ユニプロット: Q05469
RRID: AB_2835234

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
HSL Antibody detects endogenous levels of total HSL.
RRID:
AB_2835234
引用形式: Affinity Biosciences Cat# AF6403, RRID:AB_2835234.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; Hormone-sensitive lipase; HSL; LHS; Lipase hormone sensitive; LIPE; LIPS_HUMAN;

免疫原

免疫原:
Uniprot:

Q05469(LIPS_HUMAN) >>Visit HPA database.

遺伝子(ID):
LIPE(3991)
タンパク質の説明:
hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
タンパク質配列:
MEPGSKSVSRSDWQPEPHQRPITPLEPGPEKTPIAQPESKTLQGSNTQQKPASNQRPLTQQETPAQHDAESQKEPRAQQKSASQEEFLAPQKPAPQQSPYIQRVLLTQQEAASQQGPGLGKESITQQEPALRQRHVAQPGPGPGEPPPAQQEAESTPAAQAKPGAKREPSAPTESTSQETPEQSDKQTTPVQGAKSKQGSLTELGFLTKLQELSIQRSALEWKALSEWVTDSESESDVGSSSDTDSPATMGGMVAQGVKLGFKGKSGYKVMSGYSGTSPHEKTSARNHRHYQDTASRLIHNMDLRTMTQSLVTLAEDNIAFFSSQGPGETAQRLSGVFAGVREQALGLEPALGRLLGVAHLFDLDPETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQLRALVYYAQRLLVTNRPGVLFFEGDEGLTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTISIGLVSFGEHYKRNETGLSVAASSLFTSGRFAIDPELRGAEFERITQNLDVHFWKAFWNITEMEVLSSLANMASATVRVSRLLSLPPEAFEMPLTADPTLTVTISPPLAHTGPGPVLVRLISYDLREGQDSEELSSLIKSNGQRSLELWPRPQQAPRSRSLIVHFHGGGFVAQTSRSHEPYLKSWAQELGAPIISIDYSLAPEAPFPRALEECFFAYCWAIKHCALLGSTGERICLAGDSAGGNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05469 基板として

Site PTM Type Enzyme
T188 Phosphorylation
S196 Phosphorylation
S552 Phosphorylation
S565 Phosphorylation
K624 Ubiquitination
S630 Phosphorylation
S645 Phosphorylation
S649 Phosphorylation
S650 Phosphorylation
S660 Phosphorylation
Y741 Phosphorylation
T813 Phosphorylation
S825 Phosphorylation
S835 Phosphorylation
S853 Phosphorylation P17612 (PRKACA) , Q13976 (PRKG1)
S855 Phosphorylation Q14012 (CAMK1) , P17612 (PRKACA) , Q13131 (PRKAA1) , Q9UQM7 (CAMK2A)
K872 Ubiquitination
S950 Phosphorylation P17612 (PRKACA)
S951 Phosphorylation P17612 (PRKACA)
T955 Phosphorylation
T1015 Phosphorylation

研究背景

機能:

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

PTMs:

Phosphorylation by AMPK may block translocation to lipid droplets.

細胞の位置付け:

Cell membrane. Membrane>Caveola. Cytoplasm>Cytosol.
Note: Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with CAVIN1 in the adipocyte cytoplasm. Interacts with PLIN5 (By similarity).

タンパク質ファミリー:

Belongs to the 'GDXG' lipolytic enzyme family.

研究領域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

参考文献

1). Effect of salt promote the muscle triglyceride hydrolysis during dry-salting by inducing the phosphorylation of adipose tissue triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and lipid droplets splitting. Food Chemistry (PubMed: 32454271) [IF=8.8]

Application: WB    Species: Pig    Sample: adipose

Fig. 3. Western blotting analysis of ATGL, HSL and their phosphorylated protein contents in adipose tissue during processing of dry-cured meat. (A) WB image of each dry-cured group. (B) ATGL relative protein level in adipose tissue. (C) p-ATGL(S406) relative protein level in adipose tissue. (D) HSL relative protein level in adipose tissue. (E) p-HSL(Ser563) relative protein level in adipose tissue. (F) p-HSL(Ser660) relative protein level in adipose tissue.
2). MIIP downregulation drives colorectal cancer progression through inducing peri-cancerous adipose tissue browning. Cell & bioscience (PubMed: 38245780) [IF=7.5]
3). Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function. JOURNAL OF LIPID RESEARCH (PubMed: 30552289) [IF=6.5]

Application: WB    Species: pig    Sample: porcine intramuscular preadipocytes

Fig.?5.|Melatonin-activated PKA and ERK1/2 mediate lipolysis in porcine intramuscular preadipocytes. A–J: Fully differentiated adipocytes were treated with control, 1 mM of melatonin, and 1 mM of melatonin plus 10 M of 4-P-PDOT for 24 h. The expression levels of PKA (A, B), ERK1/2 (C, D), HSL (E, F), PLIN1 (G, H), and ATGL (I, J) and the phosphorylation levels of PKA (p-PKA Thr197) (A, B), ERK1/2 (p-ERK1/2 Thr202/Tyr204) (C, D), HSL (pHSL Ser660) (E, F), PLIN1 (p-PLIN1 Ser522) (G, H), and -tubulin (I, J) were evaluated by Western blotting. The results are represented as the mean ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001; n = 3).
4). Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (PubMed: 36695046) [IF=6.1]
5). The Effects of Erchen Decoction on Gut Microbiota and Lipid Metabolism Disorders in Zucker Diabetic Fatty Rats. Frontiers in Pharmacology (PubMed: 34366839) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 3 ECD improved insulin signal transduction and decreased lipolysis in WAT of ZDF rats. (A) Representative image (bar: 100 µM) and (B) number and average area of adipocytes of WAT HE staining (n = 3. Z vs. L, ****p < 0.0001; EC vs. Z, ## p < 0.01, ### p < 0.001). (C) Representative bands and (D) relative protein expression of p-IRS1/IRS1, p-AKT/AKT, p-PKA/PKA, p-HSL/HSL, and ATGL. (E) Serum FFA concentration (n = 4. Z vs. L, **p < 0.01, ***p < 0.001, ****p < 0.0001; EC vs. Z, # p < 0.05, ## p < 0.01). (B), (D), and (E) were analyzed by one-way ANOVA.
6). Sex hormone-binding globulin improves lipid metabolism and reduces inflammation in subcutaneous adipose tissue of metabolic syndrome-affected horses. Frontiers in molecular biosciences (PubMed: 38146533) [IF=5.0]
7). Effects of high-intensity interval training on adipose tissue lipolysis, inflammation, and metabolomics in aged rats. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY (PubMed: 32006095) [IF=4.5]

Application: WB    Species: rat    Sample: fat

Fig. 4 |Metabolic pathway enrichment analysis and lipolysis metabolismrelated protein and mRNA expression. Correlation analysis of 9 metabolites (a); metabolic pathway analysis in MICT versus SED (b);mRNA levels of PPAR-γ, HSL, ATGL, and TNF-α in adipose tissues (c);correlation analysis of 6 metabolites (d); metabolic pathway analysis in HIIT versus SED (e); and contents of PPAR-γ, HSL, ATGL, TNF-α, and P450scc protein (f).
8). The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice. Frontiers in Physiology (PubMed: 34588991) [IF=4.0]

Application: WB    Species: Mice    Sample: gastrocnemius

Figure 7 The expression levels of lipid metabolism-related proteins after chronic lactate and forskolin injection. (A) Western blot analysis and relative fold protein expression of lipolysis-related proteins. (B) Western blot analysis and relative fold protein expression of lipogenesis-related proteins. CP, chronic PBS treated group; CL, chronic lactate treated group; CF, chronic forskolin treated group; CD, chronic DMSO treated group; and CLF, chronic lactate and forskolin treated group. Relative expression levels were normalized to GAPDH. Three bands are used for statistics. The data are presented as the mean±SD, and significant differences among the groups were analyzed with one-way ANOVA. * p<0.05.
9). Androgens exacerbate hepatic triglyceride accumulation in rats with polycystic ovary syndrome by downregulating MTTP expression. Endocrine (PubMed: 37950821) [IF=3.7]
10). Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism. Food & Nutrition Research (PubMed: 35382382) [IF=3.3]

Application: WB    Species: mouse    Sample: skeletal muscle

Fig. 6. | Effects of sesamol intervention on the expressions of glucose and lipid metabolism-related proteins in skeletal muscle of the middle-aged mice.(e) Representative western blot images and quantification of (f) SREBP1-c, (g) p-HSL and HSL and (h) CPT1α. *P < 0.05,**P < 0.01.
もっと読みます

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.