PACSIN1 Antibody - #DF15266
製品説明
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
A830061D09Rik; Dynamin PRD-interacting protein; Dynamin proline-rich domain-interacting protein; H74; KIAA1379; MGC114042; MGC56324; mKIAA1379; OTTHUMP00000016231; PACN1_HUMAN; Pacsin1; PASCIN; Protein kinase C and casein kinase substrate in neurons 1; Protein kinase C and casein kinase substrate in neurons protein 1; SDPI; Synaptic, dynamin-associated protein I; Syndapin 1; Syndapin; Syndapin-I; zgc:114042; zgc:56324;
免疫原
A synthesized peptide derived from human PACSIN1.
- Q9BY11 PACN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSSYDEASLAPEETTDSFWEVGNYKRTVKRIDDGHRLCNDLMNCVQERAKIEKAYGQQLTDWAKRWRQLIEKGPQYGSLERAWGAIMTEADKVSELHQEVKNNLLNEDLEKVKNWQKDAYHKQIMGGFKETKEAEDGFRKAQKPWAKKMKELEAAKKAYHLACKEEKLAMTREMNSKTEQSVTPEQQKKLQDKVDKCKQDVQKTQEKYEKVLEDVGKTTPQYMENMEQVFEQCQQFEEKRLVFLKEVLLDIKRHLNLAENSSYIHVYRELEQAIRGADAQEDLRWFRSTSGPGMPMNWPQFEEWNPDLPHTTTKKEKQPKKAEGVALTNATGAVESTSQAGDRGSVSSYDRGQPYATEWSDDESGNPFGGSETNGGANPFEDDSKGVRVRALYDYDGQEQDELSFKAGDELTKLGEEDEQGWCRGRLDSGQLGLYPANYVEAI
研究背景
Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity). Binds to membranes via its F-BAR domain and mediates membrane tubulation.
Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Cytoplasm. Cell projection. Cell junction>Synapse>Synaptosome. Cell projection>Ruffle membrane. Membrane>Peripheral membrane protein. Cytoplasmic vesicle membrane>Peripheral membrane protein. Cell junction>Synapse. Cytoplasm>Cytosol. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Colocalizes with MAPT in axons. In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites (By similarity). Associates with membranes via its F-BAR domain.
Highly expressed in brain and, at much lower levels, in heart and pancreas.
The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain. DNM1 binding abolishes autoinhibition (By similarity).
Belongs to the PACSIN family.
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