MICU1 Antibody - #DF14914

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製品: MICU1 Antibody
カタログ: DF14914
タンパク質の説明: Rabbit polyclonal antibody to MICU1
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse, Rat
分子量: 54kD(Calculated).
ユニプロット: Q9BPX6

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
MICU1 Antibody detects endogenous levels of MICU1.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Allergen Hom s 4; ara CALC; atopy related autoantigen; atopy related autoantigen CALC; Atopy-related autoantigen CALC; CALC; calcium binding atopy related autoantigen 1; Calcium uptake protein 1; calcium uptake protein 1, mitochondrial; Calcium-binding atopy-related autoantigen 1; CBARA1; EFHA3; FLJ12684; MICU1; MICU1_HUMAN; mitochondrial; mitochondrial calcium uptake 1; MPXPS;

免疫原

免疫原:

A synthesized peptide derived from human MICU1.

Uniprot:

Q9BPX6(MICU1_HUMAN) >>Visit HPA database.

遺伝子(ID):
MICU1(10367)
発現特異性:
Q9BPX6 MICU1_HUMAN:

Expressed in epithelial cell lines. Strongly expressed in epidermal keratinocytes and dermal endothelial cells.

タンパク質配列:
MFRLNSLSALAELAVGSRWYHGGSQPIQIRRRLMMVAFLGASAVTASTGLLWKRAHAESPPCVDNLKSDIGDKGKNKDEGDVCNHEKKTADLAPHPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFMTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

研究背景

機能:

Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains. MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium. MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake. Acts as a gatekeeper of MCU at low concentration of calcium, preventing channel opening. Enhances MCU opening at high calcium concentration, allowing a rapid response of mitochondria to calcium signals generated in the cytoplasm. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG.

細胞の位置付け:

Mitochondrion inner membrane>Single-pass membrane protein. Mitochondrion intermembrane space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in epithelial cell lines. Strongly expressed in epidermal keratinocytes and dermal endothelial cells.

タンパク質ファミリー:

The C-helix is required for assembling the Ca(2+)-free homohexamer (PubMed:24514027). It also plays a key role in mitochondrial calcium uptake, probably by mediating interaction with MICU2 (PubMed:24503055, PubMed:24514027).

The EF-hand domains have high affinity for calcium and act as sensors of calcium levels (PubMed:23101630, PubMed:24560927).

Belongs to the MICU1 family. MICU1 subfamily.

参考文献

1). Mitochondrial calcium homeostasis mediated by estradiol contributes to atrial fibrillation protection. Biochemical and biophysical research communications, 2025 (PubMed: 40414005) [IF=2.5]

Application: IF/ICC    Species: Rat    Sample:

Fig. 1. OVX increases atrial fibrillation susceptibility and disrupts mitochondrial calcium homeostasis. (A) Representative ECG recordings from the control, AF, and AF + OVX groups, showing increased AF susceptibility in the OVX group. (B) Quantification of E2 concentration in different groups. (C) Quantification of Ca2+ content among different groups, showing a significant decrease in calcium load in the AF + OVX group. (D, E) Detection of mitochondrial membrane potential changes between groups using JC-1 monomer probe.Data are presented as mean ± SEM. Statistical significance is indicated as follows: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.(F–H) Representative of immunofluorescence staining for MICU1, NCX, and LETM1.

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