HPGD Antibody - #DF14850

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製品: HPGD Antibody
カタログ: DF14850
タンパク質の説明: Rabbit polyclonal antibody to HPGD
アプリケーション: IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse
分子量: 29kD(Calculated).
ユニプロット: P15428

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MS Report
HPLC Report
MSDS
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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
HPGD Antibody detects endogenous levels of HPGD.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

15 hydroxyprostaglandin dehydrogenase [NAD+]; 15 PGDH; 15-hydroxyprostaglandin dehydrogenase [NAD+]; 15-PGDH; 15PGDH; Hpgd; Hydroxyprostaglandin dehydrogenase 15 (NAD); NAD+ dependent 15 hydroxyprostaglandin dehydrogenase; OTTHUMP00000218960; OTTHUMP00000219016; OTTHUMP00000219018; PGDH; PGDH_HUMAN; PGDH1; PHOAR1; Prostaglandin dehydrogenase 1; SDR36C1; Short chain dehydrogenase/reductase family 36C member 1;

免疫原

免疫原:

A synthesized peptide derived from human HPGD.

Uniprot:

P15428(PGDH_HUMAN) >>Visit HPA database.

遺伝子(ID):
HPGD(3248)
発現特異性:
P15428 PGDH_HUMAN:

Detected in colon epithelium (at protein level).

タンパク質配列:
MHVNGKVALVTGAAQGIGRAFAEALLLKGAKVALVDWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQQLRDTFRKVVDHFGRLDILVNNAGVNNEKNWEKTLQINLVSVISGTYLGLDYMSKQNGGEGGIIINMSSLAGLMPVAQQPVYCASKHGIVGFTRSAALAANLMNSGVRLNAICPGFVNTAILESIEKEENMGQYIEYKDHIKDMIKYYGILDPPLIANGLITLIEDDALNGAIMKITTSKGIHFQDYDTTPFQAKTQ

研究背景

機能:

Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in colon epithelium (at protein level).

タンパク質ファミリー:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

研究領域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

参考文献

1). Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke. Aging and disease, 2023 (PubMed: 37199734) [IF=7.0]

Application: IF/ICC    Species: Mouse    Sample:

Figure 3. Subpopulation analysis of microglia clusters under homeostatic conditions. (A) Pseudotime plot (from monocle3) shows the differentiation trajectory from Mic_home to Mic_pre2 cells. (B) Line plot of the fraction of cells in the Mic_home, Mic_pre1, and Mic_pre2 subpopulations over time. (C) Expression of the ten genes with the highest Moran’s I score alongside the pseudotime trajectory. (D) Venn plot of the mutual and unique marker genes for the Mic_home, Mic_pre1, and Mic_pre2 subpopulations. (E) Volcano plot displays the differentially expressed genes between Mic_home and Mic_pre2 cells. Red dots represent the upregulated genes in the Mic_home cluster compared with the Mic_pre2 cluster and blue dots represent the downregulated genes. (F) Violin plot of the gene set variation analysis (GSVA) scores for important functional pathways of the Mic_home, Mic_pre1, and Mic_pre2 subpopulations. (G) Double immunofluorescence staining of Mic_home cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), DAPI (blue, representing cell nuclei), and Hpgd (red, representing the marker of Mic_home cells) antibodies (N=3). The yellow bar represents 100μm. (H) Double immunofluorescence staining of Mic_pre2 cells at each sampling time. Coronal brain sections are all stained with anti-Iba1(green, representing microglia), DAPI (blue, representing cell nuclei), and Wsb1 (red, representing the marker of Mic_pre2 cells) antibodies (N=3). The yellow bar represents 100μm. (I) Bar plot of the ratio of Hpgd+Iba1+ cells (green plus red) compared with all DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6). (J) Bar plot of the ratio of Wsb1+Iba1+ cells (green plus red) compared with DAPI+ cells in a 500μm2 area surrounding the infarction core (N=6).

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